Construction and Investigation of MicroRNA-mRNA Regulatory Network of Gastric Cancer with Helicobacter pylori Infection
Joint Authors
Liu, Junjie
Yang, Tianci
Gong, Peiyou
Li, Boqing
Zhou, Xiuzhi
Zhang, Lei
Yang, Ping
Source
Biochemistry Research International
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-25
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background.
Helicobacter pylori (H.
pylori) is a common human pathogen, which is closely correlated with gastric cancer (GC).
However, the mechanism of H.
pylori-related GC has not been elucidated.
This study aimed to explore the role of H.
pylori infection in GC and find biomarkers for early diagnosis of H.
pylori-related GC.
Methods.
We identified differentially expressed microRNAs (DEMs) and genes (DEGs) from the Gene Expression Omnibus (GEO) dataset, constructed microRNA-(miRNA-)mRNA expression networks, analyzed the function and signal pathway of cross-genes, analyzed the relations between cross-genes and GC prognosis with the Cancer Genome Atlas (TCGA) data, and verified the expression of cross-genes in patients with H.
pylori infection.
Results.
22 DEMs and 68 DEGs were identified in GSE197694 and GSE27411 dataset.
16 miRNAs and 509 genes were involved in the expression network, while the cross-genes of the network were mainly enriched in MAP kinase (MAPK) signaling pathway and TGF-beta signaling pathway.
Patients with higher expression of hsa-miR-196b-3p, CALML4, or SMAD6 or lower expression of PITX2 or TGFB2 had better outcomes than those with lower expression of hsa-miR-196b-3p, CALML4, or SMAD6 or higher expression of PITX2 or TGFB2 (P<0.05).
Patients with H.
pylori infection had a higher expression of hsa-miR-196b-3p and CALML4 than those without H.
pylori infection (P<0.05).
Conclusion.
The study of miRNA-mRNA expression network would provide molecular support for early diagnosis and treatment of H.
pylori-related GC.
American Psychological Association (APA)
Yang, Ping& Liu, Junjie& Yang, Tianci& Zhang, Lei& Gong, Peiyou& Li, Boqing…[et al.]. 2020. Construction and Investigation of MicroRNA-mRNA Regulatory Network of Gastric Cancer with Helicobacter pylori Infection. Biochemistry Research International،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1138457
Modern Language Association (MLA)
Yang, Ping…[et al.]. Construction and Investigation of MicroRNA-mRNA Regulatory Network of Gastric Cancer with Helicobacter pylori Infection. Biochemistry Research International No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1138457
American Medical Association (AMA)
Yang, Ping& Liu, Junjie& Yang, Tianci& Zhang, Lei& Gong, Peiyou& Li, Boqing…[et al.]. Construction and Investigation of MicroRNA-mRNA Regulatory Network of Gastric Cancer with Helicobacter pylori Infection. Biochemistry Research International. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1138457
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1138457