Comparison between Conventional Decalcification and a Microwave-Assisted Method in Bone Tissue Affected with Mycetoma
Author
Source
Biochemistry Research International
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-08-01
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Mycetoma is a lifelong granulomatous disease of subcutaneous tissues and bones.
Histopathology is a substantiated indicative method based on the assumption of a definitive diagnosis of mycetoma.
It requires efficient processing of tissues including bone decalcification.
The decalcification process must ensure complete removal of calcium and also a proper preservation of tissue and microorganisms’ staining ability.
Objectives.
To compare the conventional method used in decalcification with the microwave method using different decalcification solutions.
Different characteristics were tested, including the speed of decalcification and morphological and fungal preservation in bone tissue affected with mycetoma.
Materials and Methods.
Three decalcification solutions were employed to remove calcium from 50 bone tissue samples affected with mycetoma, including 10% neutral buffered EDTA (pH 7.4), 5% nitric acid, and 5% hydrochloric acid.
Conventional and microwave methods were used.
Haematoxylin-eosin (HE) stain, Gridley’s stain, and Grocott hexamine-silver stain were employed to evaluate the bone and fungi morphologies.
Results.
The decalcification time of the conventional method compared with the microwave method with 10% EDTA (pH 7.4) took 120 hours and 29 hours, while 5% hydrochloric acid and 5% nitric acid took 8 hours and 3 hours, separately.
Also, 10% EDTA is the best decalcifying agent for HE staining and fungal stains.
5% hydrochloric acid and 5% nitric acid can be used for fungal staining.
Conclusion.
The current study investigated the effects of different decalcifying agents as well as two decalcification procedures on the preservation of the bone structure and fungal staining, which will help to develop suitable protocols for the analyses of the bone tissue affected with mycetoma infection.
American Psychological Association (APA)
Salih, Magdi Mansour. 2020. Comparison between Conventional Decalcification and a Microwave-Assisted Method in Bone Tissue Affected with Mycetoma. Biochemistry Research International،Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1138459
Modern Language Association (MLA)
Salih, Magdi Mansour. Comparison between Conventional Decalcification and a Microwave-Assisted Method in Bone Tissue Affected with Mycetoma. Biochemistry Research International No. 2020 (2020), pp.1-6.
https://search.emarefa.net/detail/BIM-1138459
American Medical Association (AMA)
Salih, Magdi Mansour. Comparison between Conventional Decalcification and a Microwave-Assisted Method in Bone Tissue Affected with Mycetoma. Biochemistry Research International. 2020. Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1138459
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1138459