The Effect of L-Carnitine, Hypotaurine, and Taurine Supplementation on the Quality of Cryopreserved Chicken Semen
Joint Authors
Partyka, Agnieszka
Rodak, Olga
Bajzert, Joanna
Kochan, Joanna
Niżański, Wojciech
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-04-24
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The objective of this study was to investigate the effect of L-carnitine (LC), hypotaurine (HT), and taurine (T) on the quality of frozen-thawed chicken semen.
Pooled semen samples were divided into seven aliquots (control, 1 mM LC, 5 mM LC, 1 mM HT, 10 mM HT, 1 mM T, and 10 mM T) and subjected to cryopreservation.
Postthaw sperm motility was determined by IVOS system and sperm characteristics were assessed with fluorochromes and flow cytometry.
The highest sperm motility and the highest percentage of viable sperm were in the HT1 group (P<0.01 and P<0.05) following cryopreservation.
After thawing, we observed a higher percentage of sperm without apoptosis and membrane reorganization changes in the LC1 and T1 group when compared to the control (P<0.05).
There was a higher percentage of live sperm without lipid peroxidation (LPO) in all treatments (P<0.01; P<0.05), when compared to the control group.
The percentage of sperm with high mitochondrial potential significantly increased with LC1, T1, and T10 (P<0.05).
Supplementation of the diluent with LC1, LC5, and T1 significantly (P<0.05) reduced DNA susceptibility to fragmentation, compared to the control and HT1 groups.
These results indicate that the addition of examined antioxidants improves the quality of cryopreserved chicken semen.
American Psychological Association (APA)
Partyka, Agnieszka& Rodak, Olga& Bajzert, Joanna& Kochan, Joanna& Niżański, Wojciech. 2017. The Effect of L-Carnitine, Hypotaurine, and Taurine Supplementation on the Quality of Cryopreserved Chicken Semen. BioMed Research International،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1138493
Modern Language Association (MLA)
Partyka, Agnieszka…[et al.]. The Effect of L-Carnitine, Hypotaurine, and Taurine Supplementation on the Quality of Cryopreserved Chicken Semen. BioMed Research International No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1138493
American Medical Association (AMA)
Partyka, Agnieszka& Rodak, Olga& Bajzert, Joanna& Kochan, Joanna& Niżański, Wojciech. The Effect of L-Carnitine, Hypotaurine, and Taurine Supplementation on the Quality of Cryopreserved Chicken Semen. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1138493
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1138493