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Urinary Exosomal miR-193a Can Be a Potential Biomarker for the Diagnosis of Primary Focal Segmental Glomerulosclerosis in Children
Joint Authors
Huang, Zhibin
Zhang, Yong
Zhou, Jianhua
Zhang, Yu
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-29
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Background.
Glomerular upregulation of miR-193a has been detected in primary focal segmental glomerulosclerosis (FSGS) but not in other glomerular diseases.
We aimed to isolate exosomes from urine of children with primary FSGS and to assess the diagnostic potential of urinary exosomal miR-193a for primary FSGS.
Methods.
The first morning urine samples were collected from children with primary FSGS (n=8) and minimal change disease (MCD, n=5).
Isolated urinary exosomes were confirmed by electron microscopy and Western blotting.
Urinary exosomal microRNA was extracted, and the expression levels of exosomal miR-193a were quantified by real-time PCR.
The diagnosis value of urinary exosomal miR-193a levels for primary FSGS was evaluated by ROC analysis.
Results.
The isolated vesicles were qualitatively compatible with exosomes.
The levels of urinary exosomal miR-193a were significantly higher in children with primary FSGS than those in children with MCD.
Moreover, the area under the ROC for the diagnosis of primary FSGS using urinary exosomal miR-193a was 0.85.
Conclusions.
A significant increase in the levels of urinary exosomal miR-193a in primary FSGS patients compared to those in MCD ones was observed.
This study suggests that urinary exosomal miR-193a may be a new noninvasive biomarker for the diagnosis of primary FSGS.
American Psychological Association (APA)
Huang, Zhibin& Zhang, Yong& Zhou, Jianhua& Zhang, Yu. 2017. Urinary Exosomal miR-193a Can Be a Potential Biomarker for the Diagnosis of Primary Focal Segmental Glomerulosclerosis in Children. BioMed Research International،Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1138497
Modern Language Association (MLA)
Huang, Zhibin…[et al.]. Urinary Exosomal miR-193a Can Be a Potential Biomarker for the Diagnosis of Primary Focal Segmental Glomerulosclerosis in Children. BioMed Research International No. 2017 (2017), pp.1-6.
https://search.emarefa.net/detail/BIM-1138497
American Medical Association (AMA)
Huang, Zhibin& Zhang, Yong& Zhou, Jianhua& Zhang, Yu. Urinary Exosomal miR-193a Can Be a Potential Biomarker for the Diagnosis of Primary Focal Segmental Glomerulosclerosis in Children. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1138497
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1138497