Selective Removal of Hemoglobin from Blood Using Hierarchical Copper Shells Anchored to Magnetic Nanoparticles
Joint Authors
Liu, Youxun
Huang, Juan
Wang, Yaokun
Yan, Mingyang
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-02-21
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Hierarchical copper shells anchored on magnetic nanoparticles were designed and fabricated to selectively deplete hemoglobin from human blood by immobilized metal affinity chromatography.
Briefly, CoFe2O4 nanoparticles coated with polyacrylic acid were first synthesized by a one-pot solvothermal method.
Hierarchical copper shells were then deposited by immobilizing Cu2+ on nanoparticles and subsequently by reducing between the solid CoFe2O4@COOH and copper solution with NaBH4.
The resulting nanoparticles were characterized by scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectrometry, X-ray photoelectron spectroscopy, and vibrating sample magnetometry.
The particles were also tested against purified bovine hemoglobin over a range of pH, contact time, and initial protein concentration.
Hemoglobin adsorption followed pseudo-second-order kinetics and reached equilibrium in 90 min.
Isothermal data also fit the Langmuir model well, with calculated maximum adsorption capacity 666 mg g−1.
Due to the high density of Cu2+ on the shell, the nanoparticles efficiently and selectively deplete hemoglobin from human blood.
Taken together, the results demonstrate that the particles with hierarchical copper shells effectively remove abundant, histidine-rich proteins, such as hemoglobin from human blood, and thereby minimize interference in diagnostic and other assays.
American Psychological Association (APA)
Liu, Youxun& Wang, Yaokun& Yan, Mingyang& Huang, Juan. 2017. Selective Removal of Hemoglobin from Blood Using Hierarchical Copper Shells Anchored to Magnetic Nanoparticles. BioMed Research International،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1138502
Modern Language Association (MLA)
Liu, Youxun…[et al.]. Selective Removal of Hemoglobin from Blood Using Hierarchical Copper Shells Anchored to Magnetic Nanoparticles. BioMed Research International No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1138502
American Medical Association (AMA)
Liu, Youxun& Wang, Yaokun& Yan, Mingyang& Huang, Juan. Selective Removal of Hemoglobin from Blood Using Hierarchical Copper Shells Anchored to Magnetic Nanoparticles. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1138502
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1138502