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Variety of RNAs in Peripheral Blood Cells, Plasma, and Plasma Fractions
Joint Authors
Semenov, Dmitry V.
Kuligina, Elena V.
Richter, Vladimir A.
Savelyeva, Anna V.
Bariakin, Dmitry N.
Kozlov, Vadim V.
Ryabchikova, Elena I.
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-03
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Human peripheral blood contains RNA in cells and in extracellular membrane vesicles, microvesicles and exosomes, as well as in cell-free ribonucleoproteins.
Circulating mRNAs and noncoding RNAs, being internalized, possess the ability to modulate vital processes in recipient cells.
In this study, with SOLiD sequencing technology, we performed identification, classification, and quantification of RNAs from blood fractions: cells, plasma, plasma vesicles pelleted at 16,000g and 160,000g, and vesicle-depleted plasma supernatant of healthy donors and non-small cell lung cancer (NSCLC) patients.
It was determined that 16,000g blood plasma vesicles were enriched with cell-free mitochondria and with a set of mitochondrial RNAs.
The variable RNA set of blood plasma 160,000g pellets reflected the prominent contribution of U1, U5, and U6 small nuclear RNAs’ fragments and at the same time was characterized by a remarkable depletion of small nucleolar RNAs.
Besides microRNAs, the variety of fragments of mRNAs and snoRNAs dominated in the set of circulating RNAs differentially expressed in blood fractions of NSCLC patients.
Taken together, our data emphasize that not only extracellular microRNAs but also circulating fragments of messenger and small nuclear/nucleolar RNAs represent prominent classes of circulating regulatory ncRNAs as well as promising circulating biomarkers for the development of disease diagnostic approaches.
American Psychological Association (APA)
Savelyeva, Anna V.& Kuligina, Elena V.& Bariakin, Dmitry N.& Kozlov, Vadim V.& Ryabchikova, Elena I.& Richter, Vladimir A.…[et al.]. 2017. Variety of RNAs in Peripheral Blood Cells, Plasma, and Plasma Fractions. BioMed Research International،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1138560
Modern Language Association (MLA)
Savelyeva, Anna V.…[et al.]. Variety of RNAs in Peripheral Blood Cells, Plasma, and Plasma Fractions. BioMed Research International No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1138560
American Medical Association (AMA)
Savelyeva, Anna V.& Kuligina, Elena V.& Bariakin, Dmitry N.& Kozlov, Vadim V.& Ryabchikova, Elena I.& Richter, Vladimir A.…[et al.]. Variety of RNAs in Peripheral Blood Cells, Plasma, and Plasma Fractions. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1138560
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1138560