Avβ3 Single-Stranded DNA Aptamer Attenuates Vascular Smooth Muscle Cell Proliferation and Migration via Ras-PI3KMAPK Pathway
Joint Authors
Wu, Hong-Bing
Wang, Zhi-Wei
Shi, Feng
Ren, Zong-Li
Li, Luo-Cheng
Hu, Xiao-Ping
Hu, Rui
Li, Bo-Wen
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-01-21
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Objectives.
To observe the effect of avβ3 single-stranded (ss) DNA on proliferation and migration of vascular smooth muscle cells (VSMCs) and its potential mechanism.
Background.
Percutaneous transluminal coronary angioplasty (PTCA) is currently the preferred method for the treatment of coronary heart disease.
However, vascular restenosis still occurs after PTCA treatment, severely affecting the clinical efficacy of PTCA.
Integrin avβ3, which is widely expressed on various cell surfaces, plays an important role in the proliferation and migration of VSMCs.
Methods.
In this experiment, we used systematic evolution of ligands by exponential enrichment (SELEX) to screen out avβ3 ssDNA, which has high affinity and specificity to the avβ3 protein.
MTT, Transwell, and cell scratch assays were carried out to examine the effect of avβ3 ssDNA on the proliferation and migration of VSMCs.
Flow cytometry was performed to detect apoptosis and cell cycle progression.
The effect of avβ3 ssDNA on the Ras-phosphatidylinositol-4,5-bisphosphate 3-kinase/mitogen-activated protein kinase (PI3K/MAPK) signaling pathway was evaluated by quantitative reverse transcription polymerase chain reaction and western blot.
Results.
In the present study, we found that avβ3 ssDNA significantly decreased the expression of osteopontin, focal adhesion kinase, Ras, p-PI3K, and p-MAPK at both mRNA and protein levels (P<0.05).
Avβ3 ssDNA also inhibited VSMC proliferation and migration while promoting apoptosis (P<0.05), as demonstrated by the upregulation of the proapoptotic proteins Bax and active caspase 3 (P<0.05).
Conclusions.
The findings suggest that avβ3 ssDNA inhibited the proliferation and migration of VSMCs by suppressing the activation of Ras-PI3K/MAPK signaling.
American Psychological Association (APA)
Wu, Hong-Bing& Wang, Zhi-Wei& Shi, Feng& Ren, Zong-Li& Li, Luo-Cheng& Hu, Xiao-Ping…[et al.]. 2020. Avβ3 Single-Stranded DNA Aptamer Attenuates Vascular Smooth Muscle Cell Proliferation and Migration via Ras-PI3KMAPK Pathway. Cardiovascular Therapeutics،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1138622
Modern Language Association (MLA)
Wu, Hong-Bing…[et al.]. Avβ3 Single-Stranded DNA Aptamer Attenuates Vascular Smooth Muscle Cell Proliferation and Migration via Ras-PI3KMAPK Pathway. Cardiovascular Therapeutics No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1138622
American Medical Association (AMA)
Wu, Hong-Bing& Wang, Zhi-Wei& Shi, Feng& Ren, Zong-Li& Li, Luo-Cheng& Hu, Xiao-Ping…[et al.]. Avβ3 Single-Stranded DNA Aptamer Attenuates Vascular Smooth Muscle Cell Proliferation and Migration via Ras-PI3KMAPK Pathway. Cardiovascular Therapeutics. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1138622
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1138622