Integrative Analysis of miRNA and mRNA Expression Profiles in Calcium Oxalate Nephrolithiasis Rat Model
Joint Authors
Wu, Wenqi
Lan, Chuangxin
Chen, Dong
Liang, Xiongfa
Huang, Jian
Zeng, Tao
Duan, Xiaolu
Chen, Kang
Lai, Yongchang
Yang, Dong
Li, Shujue
Jiang, Chonghe
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-12-17
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The microRNA (miRNA) expression profiles and their biological functions in calcium oxalate nephrolithiasis remain unclear.
In this study, we investigate the miRNA and mRNA expression profiles of kidney tissues in calcium oxalate stone rats.
16 Sprague Dawley rats were divided into control group and stone-forming group.
24-hour urine samples and kidney tissues were collected for biochemical and histological determination after 4 weeks.
MiRNA and mRNA microarray were applied to evaluate the miRNA and mRNA expression profiles.
To validate the microarray results, the quantitative real-time PCR (qRT-PCR) was performed.
A total of 38 miRNAs and 2728 mRNAs were significantly and differentially expressed in kidney tissues of stone-forming group versus control group.
Gene Ontology (GO) analysis revealed that most of the target genes were enriched in terms of oxidation reduction, ion transport, inflammatory response, and response to wounding.
Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of these targets highlights their critical role in cytokine-cytokine receptor interaction, gap junction, and chemokine signaling pathway.
Furthermore, the reliability of the microarray-based results was confirmed by using qRT-PCR determination.
The miRNA and mRNA expressions in calcium oxalate stone rat kidneys might provide a basis for further research on urolithiasis mechanism.
American Psychological Association (APA)
Lan, Chuangxin& Chen, Dong& Liang, Xiongfa& Huang, Jian& Zeng, Tao& Duan, Xiaolu…[et al.]. 2017. Integrative Analysis of miRNA and mRNA Expression Profiles in Calcium Oxalate Nephrolithiasis Rat Model. BioMed Research International،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1139021
Modern Language Association (MLA)
Lan, Chuangxin…[et al.]. Integrative Analysis of miRNA and mRNA Expression Profiles in Calcium Oxalate Nephrolithiasis Rat Model. BioMed Research International No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1139021
American Medical Association (AMA)
Lan, Chuangxin& Chen, Dong& Liang, Xiongfa& Huang, Jian& Zeng, Tao& Duan, Xiaolu…[et al.]. Integrative Analysis of miRNA and mRNA Expression Profiles in Calcium Oxalate Nephrolithiasis Rat Model. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1139021
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1139021