Protective Effect of Thalidomide on 2,4,6-Trinitrobenzenesulfonic Acid-Induced Experimental Colitis in Rats via the Inhibition of T Helper 17 Cells
Joint Authors
Jin, Shuang
Xie, Ying
Yan, Bo
Li, Dandan
Luan, Xiaoshuang
Tian, Feng
Source
Canadian Journal of Gastroenterology and Hepatology
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-20
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Objective.
To observe the effects of thalidomide on 2,4,6-trinitrobenzenesulfonic acid- (TNBS-) induced experimental colitis in rats and to explore the possible mechanism of thalidomide in the treatment of CD.
Methods.
Forty SD rats were randomly assigned into a healthy control group and TNBS-induced colitis groups, including an untreated TNBS-induced colitis group, a low-dose thalidomide group, and a high-dose thalidomide group, with 10 rats in each.
After 7 days, the disease activity index (DAI), colon macroscopic damage index (CMDI), and tissue damage index (TDI) were evaluated.
The colonic protein and mRNA expression levels of interleukin-6 (IL-6), IL-17, IL-23, and retinoic acid receptor-related orphan nuclear receptor gamma t (RORγt) were determined using immunohistochemistry, western blot, and qRT-PCR.
Results.
Relative to the untreated TNBS-induced colitis group, the DAI, CMDI, and TDI were all reduced following the administration of thalidomide.
Analytical testing (immunohistochemistry, western blot, and qRT-PCR) shows that IL-6, IL-17, IL-23, and RORγt protein and mRNA expression levels were significantly reduced by thalidomide (p<0.05 for all) and that these levels were significantly lower in the high-dose thalidomide group than in the low-dose thalidomide group (p<0.05 for all).
Conclusions.
Thalidomide effectively alleviated the symptoms and intestinal inflammatory injury induced by TNBS in rats, the effect of which was dose-dependent.
The underlying mechanism may be a reduction in the expression levels of IL-6, IL-17, IL-23, and RORγt in colonic tissue and then subsequent inhibition of the differentiation and function of Th17 cells, thus further alleviating the intestinal inflammatory response.
American Psychological Association (APA)
Xie, Ying& Li, Dandan& Luan, Xiaoshuang& Jin, Shuang& Yan, Bo& Tian, Feng. 2020. Protective Effect of Thalidomide on 2,4,6-Trinitrobenzenesulfonic Acid-Induced Experimental Colitis in Rats via the Inhibition of T Helper 17 Cells. Canadian Journal of Gastroenterology and Hepatology،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1139059
Modern Language Association (MLA)
Xie, Ying…[et al.]. Protective Effect of Thalidomide on 2,4,6-Trinitrobenzenesulfonic Acid-Induced Experimental Colitis in Rats via the Inhibition of T Helper 17 Cells. Canadian Journal of Gastroenterology and Hepatology No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1139059
American Medical Association (AMA)
Xie, Ying& Li, Dandan& Luan, Xiaoshuang& Jin, Shuang& Yan, Bo& Tian, Feng. Protective Effect of Thalidomide on 2,4,6-Trinitrobenzenesulfonic Acid-Induced Experimental Colitis in Rats via the Inhibition of T Helper 17 Cells. Canadian Journal of Gastroenterology and Hepatology. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1139059
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1139059