Optimization of the Time Window of Interest in Ovariectomized Imprinting Control Region Mice for Antiosteoporosis Research
Joint Authors
Zhang, Yue
Yuan, Xiaomei
Bi, Yanan
Zhou, Kun
Zhang, Pan-yang
Song, Lei
Liu, Ying
Huang, Ju-yang
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-10-08
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
This study was performed to determine the optimal window of time during which the properties of osteoporosis are obvious and to explore the best region of interest for microstructural evaluation in antiosteoporosis research in an ovariectomized mouse model by examining changes in micro-computed tomography parameters and serum indices.
Ovariectomized mice and sham-operated mice were randomly divided into five groups.
At the end of the 4th, 8th, 12th, 16th, and 20th weeks after ovariectomy, the microstructure of the proximal tibia and distal femur was scanned by micro-computed tomography and blood samples were collected to detect serum biochemical indicators including alkaline phosphatase, osteocalcin, N-terminal propeptide of type I procollagen (P1NP), and C-terminal telopeptide fragment of type I collagen (CTX1).
The trabecular number and connectivity density decreased while the trabecular thickness and trabecular separation increased, indicating substantial changes in the trabecular microstructure of both the tibia and femur and significant changes in bone turnover after ovariectomy, as indicated by lower levels of serum alkaline phosphatase, osteocalcin, and P1NP and higher level of CTX1 in the ovariectomy than sham group.
The proximal tibia from weeks 8 to 16 after ovariectomy was optimal for osteoporosis research in this model.
American Psychological Association (APA)
Song, Lei& Bi, Yanan& Zhang, Pan-yang& Yuan, Xiaomei& Liu, Ying& Zhang, Yue…[et al.]. 2017. Optimization of the Time Window of Interest in Ovariectomized Imprinting Control Region Mice for Antiosteoporosis Research. BioMed Research International،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1139083
Modern Language Association (MLA)
Song, Lei…[et al.]. Optimization of the Time Window of Interest in Ovariectomized Imprinting Control Region Mice for Antiosteoporosis Research. BioMed Research International No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1139083
American Medical Association (AMA)
Song, Lei& Bi, Yanan& Zhang, Pan-yang& Yuan, Xiaomei& Liu, Ying& Zhang, Yue…[et al.]. Optimization of the Time Window of Interest in Ovariectomized Imprinting Control Region Mice for Antiosteoporosis Research. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1139083
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1139083