Microarray Analysis of Circular RNA Expression Profile Associated with 5-Fluorouracil-Based Chemoradiation Resistance in Colorectal Cancer Cells
Joint Authors
Xiong, Wei
Ai, Yi-Qin
Li, Yun-Fen
Ye, Qing
Chen, Zheng-Ting
Qin, Ji-Yong
Liu, Qiu-Yan
Wang, Hong
Ju, Yun-He
Li, Wen-Hui
Li, Yun-Feng
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-06-01
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Preoperative 5-fluorouracil- (5-FU-) based chemoradiotherapy is a standard treatment for locally advanced colorectal cancer (CRC).
However, the effect of 5-FU-based chemoradiotherapy on CRC is limited due to the development of chemoradiation resistance (CRR), and the molecular mechanisms underlying this resistance are yet to be investigated.
Recently, circular RNAs (circRNAs), which can function as microRNA sponges, were found to be involved in the development of several cancers.
In this study, we focused on clarifying the modulation of the expression profiles of circRNAs in CRR.
Microarray analysis identified 71 circRNAs differentially expressed in chemoradiation-resistant CRC cells.
Among them, 47 were upregulated and 24 were downregulated by more than twofold.
Furthermore, expression modulation of five representative circRNAs was validated by quantitative reverse transcription PCR (qRT-PCR).
Moreover, these modulated circRNAs were predicted to interact with 355 miRNAs.
Furthermore, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that the most modulated circRNAs regulate several cancers and cancer-related pathways, and the possible mechanism underlying CRR was discussed.
This is the first report revealing the circRNA modulations in 5-FU chemoradiation-resistant CRC cells by microarray.
The study provided a useful database for further understanding CRR and presents potential targets to overcome CRR in CRC.
American Psychological Association (APA)
Xiong, Wei& Ai, Yi-Qin& Li, Yun-Fen& Ye, Qing& Chen, Zheng-Ting& Qin, Ji-Yong…[et al.]. 2017. Microarray Analysis of Circular RNA Expression Profile Associated with 5-Fluorouracil-Based Chemoradiation Resistance in Colorectal Cancer Cells. BioMed Research International،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1139086
Modern Language Association (MLA)
Xiong, Wei…[et al.]. Microarray Analysis of Circular RNA Expression Profile Associated with 5-Fluorouracil-Based Chemoradiation Resistance in Colorectal Cancer Cells. BioMed Research International No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1139086
American Medical Association (AMA)
Xiong, Wei& Ai, Yi-Qin& Li, Yun-Fen& Ye, Qing& Chen, Zheng-Ting& Qin, Ji-Yong…[et al.]. Microarray Analysis of Circular RNA Expression Profile Associated with 5-Fluorouracil-Based Chemoradiation Resistance in Colorectal Cancer Cells. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1139086
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1139086