Paeoniflorin, the Main Active Ingredient of Shuyu Capsule, Inhibits Cav1.2 and Regulates CalmodulinCalmodulin-Dependent Protein Kinase II Signalling
Joint Authors
Wei, Sheng
Sun, Peng
Wang, Jieqiong
Li, Fang
Gao, Dongmei
Song, Chunhong
Qiao, Mingqi
Yu, Yanhong
Wang, Meiyan
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-12-06
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The aim of this study was to explore the mechanism underlying the antidepression activity of paeoniflorin, the main active ingredient of paeony extract and Shuyu capsules, and determine its effect on the calmodulin/calmodulin-dependent protein kinase II (CaM/CaMKII) signalling pathway and on the possible target, the voltage-gated calcium channel (Cav).
Rats at the nonacceptance stage were selected for premenstrual syndrome (PMS) depression modelling.
Behavioural assays were used for model testing.
Rats were given Shuyu capsules, paeony extract, and bupleurum.
Western blot analysis was used to assess the expression levels of calcium voltage-gated channel subunit alpha 1 C (CACNA1C), brain-derived neurotrophic factor, and CaM/CaMKII signalling pathway proteins.
Intracellular Ca2+ concentration in CHO cell line was measured using Fluo-4-AM and whole-cell patch clamps.
The PMS depression model was successfully established and demonstrated that Shuyu can mitigate depressive behaviour in a rat PMS model.
Paeony extract did not affect CACNA1C protein expression in rat hippocampi but did affect Cav1.2-mediated CaM/CaMKII signalling pathways.
Paeoniflorin significantly inhibited KCl-induced increases in intracellular Ca2+ concentration and Cav1.2 current density.
Further, it may function via the CaM/CaMKII pathway and its downstream signalling molecules by regulating Cav1.2, thus playing an important role in the treatment and alleviation of affective disorders.
American Psychological Association (APA)
Song, Chunhong& Wang, Jieqiong& Gao, Dongmei& Yu, Yanhong& Li, Fang& Wei, Sheng…[et al.]. 2017. Paeoniflorin, the Main Active Ingredient of Shuyu Capsule, Inhibits Cav1.2 and Regulates CalmodulinCalmodulin-Dependent Protein Kinase II Signalling. BioMed Research International،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1139091
Modern Language Association (MLA)
Song, Chunhong…[et al.]. Paeoniflorin, the Main Active Ingredient of Shuyu Capsule, Inhibits Cav1.2 and Regulates CalmodulinCalmodulin-Dependent Protein Kinase II Signalling. BioMed Research International No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1139091
American Medical Association (AMA)
Song, Chunhong& Wang, Jieqiong& Gao, Dongmei& Yu, Yanhong& Li, Fang& Wei, Sheng…[et al.]. Paeoniflorin, the Main Active Ingredient of Shuyu Capsule, Inhibits Cav1.2 and Regulates CalmodulinCalmodulin-Dependent Protein Kinase II Signalling. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1139091
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1139091