Expression of Cadherin-17 Promotes Metastasis in a Highly Bone Marrow Metastatic Murine Breast Cancer Model
Joint Authors
Akimitsu, Nobuyoshi
Furihata, Mutsuo
Okada, Tomoko
Kurabayashi, Atsushi
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-19
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
We previously established 4T1E/M3 highly bone marrow metastatic mouse breast cancer cells through in vivo selection of 4T1 cells.
But while the incidence of bone marrow metastasis of 4T1E/M3 cells was high (~80%) when injected intravenously to mice, it was rather low (~20%) when injected subcutaneously.
Therefore, using 4T1E/M3 cells, we carried out further in vitro and in vivo selection steps to establish FP10SC2 cells, which show a very high incidence of metastasis to lungs (100%) and spines (85%) after subcutaneous injection into mice.
qRT-PCR and western bolt analysis revealed that cadherin-17 gene and protein expression were higher in FP10SC2 cells than in parental 4T1E/M3 cells.
In addition, immunostaining revealed the presence of cadherin-17 at sites of bone marrow and lung metastasis after subcutaneous injection of FP10SC2 cells into mice.
Suppressing cadherin-17 expression in FP10SC2 cells using RNAi dramatically decreased the cells’ anchorage-independent growth and migration in vitro and their metastasis to lung and bone marrow in vivo.
These findings suggest that cadherin-17 plays a crucial role in mediating breast cancer metastasis to bone marrow.
American Psychological Association (APA)
Okada, Tomoko& Kurabayashi, Atsushi& Akimitsu, Nobuyoshi& Furihata, Mutsuo. 2017. Expression of Cadherin-17 Promotes Metastasis in a Highly Bone Marrow Metastatic Murine Breast Cancer Model. BioMed Research International،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1139101
Modern Language Association (MLA)
Okada, Tomoko…[et al.]. Expression of Cadherin-17 Promotes Metastasis in a Highly Bone Marrow Metastatic Murine Breast Cancer Model. BioMed Research International No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1139101
American Medical Association (AMA)
Okada, Tomoko& Kurabayashi, Atsushi& Akimitsu, Nobuyoshi& Furihata, Mutsuo. Expression of Cadherin-17 Promotes Metastasis in a Highly Bone Marrow Metastatic Murine Breast Cancer Model. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1139101
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1139101