Downregulation of miR-214-3p May Contribute to Pathogenesis of Ulcerative Colitis via Targeting STAT6
Joint Authors
Li, Jin-an
Wang, Yong-duo
Wang, Kui
Wang, Zhen-lan
Jia, Dao-yong
Yang, Bi-ying
Xiong, Chang-bing
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-02
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
MicroRNAs (miRs) are small noncoding RNA molecules and recently have demonstrated that altered expression and functions are their tight association with ulcerative colitis (UC).
Previous microarray study reported that miR-214 was downregulated in the sigmoid colon of patients with active UC, but the roles of miR-214 in the pathogenesis of UC remain to be elucidated.
In this study, significant lower level of miR-214-3p and higher level of STAT6 in the intestinal mucosa of active UC patients compared with the health controls were confirmed by quantitative real-time PCR.
Results of luciferase reporter assays and western blot demonstrated that miR-214-3p directly targets STAT6 and negatively regulates the expression of STAT6 at the posttranscriptional level.
Furthermore, the expression of miR-214-3p was decreased in TNF-α treated HT29 cells and STAT6 protein level was increased in a time-dependent manner.
Silenced STAT6 and upregulation of miR-214-3p could decrease the level of INF-γ in TNF-α treated HT29 cells.
Additionally, the results of the present study indicate that miR-214-3p and STAT6 axis may be a novel therapeutic target for intestinal inflammation of patients with active UC.
American Psychological Association (APA)
Li, Jin-an& Wang, Yong-duo& Wang, Kui& Wang, Zhen-lan& Jia, Dao-yong& Yang, Bi-ying…[et al.]. 2017. Downregulation of miR-214-3p May Contribute to Pathogenesis of Ulcerative Colitis via Targeting STAT6. BioMed Research International،Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1139115
Modern Language Association (MLA)
Li, Jin-an…[et al.]. Downregulation of miR-214-3p May Contribute to Pathogenesis of Ulcerative Colitis via Targeting STAT6. BioMed Research International No. 2017 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-1139115
American Medical Association (AMA)
Li, Jin-an& Wang, Yong-duo& Wang, Kui& Wang, Zhen-lan& Jia, Dao-yong& Yang, Bi-ying…[et al.]. Downregulation of miR-214-3p May Contribute to Pathogenesis of Ulcerative Colitis via Targeting STAT6. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1139115
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1139115