Effects of Bacillus Serine Proteases on the Bacterial Biofilms

Joint Authors

Sharipova, Margarita
Mardanova, Ayslu
Mitrofanova, Olga
Evtugyn, Vladimir
Bogomolnaya, Lydia

Source

BioMed Research International

Issue

Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2017-08-21

Country of Publication

Egypt

No. of Pages

10

Main Subjects

Medicine

Abstract EN

Serratia marcescens is an emerging opportunistic pathogen responsible for many hospital-acquired infections including catheter-associated bacteremia and urinary tract and respiratory tract infections.

Biofilm formation is one of the mechanisms employed by S.

marcescens to increase its virulence and pathogenicity.

Here, we have investigated the main steps of the biofilm formation by S.

marcescens SR 41-8000.

It was found that the biofilm growth is stimulated by the nutrient-rich environment.

The time-course experiments showed that S.

marcescens cells adhere to the surface of the catheter and start to produce extracellular polymeric substances (EPS) within the first 2 days of growth.

After 7 days, S.

marcescens biofilms maturate and consist of bacterial cells embedded in a self-produced matrix of hydrated EPS.

In this study, the effect of Bacillus pumilus 3-19 proteolytic enzymes on the structure of 7-day-old S.

marcescens biofilms was examined.

Using quantitative methods and scanning electron microscopy for the detection of biofilm, we demonstrated a high efficacy of subtilisin-like protease and glutamyl endopeptidase in biofilm removal.

Enzymatic treatment resulted in the degradation of the EPS components and significant eradication of the biofilms.

American Psychological Association (APA)

Mitrofanova, Olga& Mardanova, Ayslu& Evtugyn, Vladimir& Bogomolnaya, Lydia& Sharipova, Margarita. 2017. Effects of Bacillus Serine Proteases on the Bacterial Biofilms. BioMed Research International،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1139117

Modern Language Association (MLA)

Mitrofanova, Olga…[et al.]. Effects of Bacillus Serine Proteases on the Bacterial Biofilms. BioMed Research International No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1139117

American Medical Association (AMA)

Mitrofanova, Olga& Mardanova, Ayslu& Evtugyn, Vladimir& Bogomolnaya, Lydia& Sharipova, Margarita. Effects of Bacillus Serine Proteases on the Bacterial Biofilms. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1139117

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1139117