Characterization of Integrons and Antimicrobial Resistance in Escherichia coli Sequence Type 131 Isolates
Joint Authors
Huang, Jiangqing
Lan, Fangjun
Lu, Yanfang
Li, Bin
Source
Canadian Journal of Infectious Diseases and Medical Microbiology
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-02-24
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Background.
Escherichia coli sequence type 131 (ST131) is an important multidrug-resistant extraintestinal pathogen, which can cause many kinds of infections.
Integrons may play a crucial role in the dissemination of antibiotic resistance genes.
The purpose of this study was to characterize the prevelance of integrons among E.
coli ST131 strains in China.
Methods.
Eighty-three E.
coli ST131 isolates were used in this study.
The antibiotic susceptibility test was performed by the disk diffusion method.
The presence and characterization of class 1, 2, and 3 integrons, as well as promotor of gene cassettes and other antimicrobial resistance genes, were detected by PCR and DNA sequencing.
Transfer of integrons was carried out using a broth culture mating method.
Clonal relatedness of E.
coli ST131 isolates was analyzed by PFGE.
Results.
Overall, 26.5% (22/83) of the E.
coli ST131 isolates carried class 1 integrons.
Class 2 and 3 integrons were not found in this study.
Two types of gene cassette arrays were demonstrated in this study and were as follows: dfrA17-aadA5 and aac(6′)-Ib-cr-cmlA5.
Only one type of Pc promoter variant was detected among 22 integron-positive isolates (PcW).
In vivo transfer of integron was successful for 9 of integron-positive E.
coli ST131 isolates harboring resistance gene cassettes.
Results of PFGE demonstrated that the integron-positive E.
coli ST131 isolates were grouped into 12 different PFGE clusters.
Conclusions.
Our study showed a low prevalence of integrons was detected in E.
coli ST131.
Continued surveillance of this mobile genetic element should be performed to study the evolution of antibiotic resistance among E.
coli ST131.
American Psychological Association (APA)
Huang, Jiangqing& Lan, Fangjun& Lu, Yanfang& Li, Bin. 2020. Characterization of Integrons and Antimicrobial Resistance in Escherichia coli Sequence Type 131 Isolates. Canadian Journal of Infectious Diseases and Medical Microbiology،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1139122
Modern Language Association (MLA)
Huang, Jiangqing…[et al.]. Characterization of Integrons and Antimicrobial Resistance in Escherichia coli Sequence Type 131 Isolates. Canadian Journal of Infectious Diseases and Medical Microbiology No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1139122
American Medical Association (AMA)
Huang, Jiangqing& Lan, Fangjun& Lu, Yanfang& Li, Bin. Characterization of Integrons and Antimicrobial Resistance in Escherichia coli Sequence Type 131 Isolates. Canadian Journal of Infectious Diseases and Medical Microbiology. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1139122
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1139122