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Serum Proteome Alterations in Patients with Cognitive Impairment after Traumatic Brain Injury Revealed by iTRAQ-Based Quantitative Proteomics
Joint Authors
Peng, Weijun
Wang, Yang
Xiong, Xin-Gui
Wang, Zhe
Xia, Zian
Zhang, Chunhu
Liang, Qinghua
Huang, Wei
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-30
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Background.
Cognitive impairment is the leading cause of traumatic brain injury- (TBI-) related disability; however, the underlying pathogenesis of this dysfunction is not completely understood.
Methods.
Using an isobaric tagging for relative and absolute quantitation- (iTRAQ-) based quantitative proteomic approach, serum samples from healthy control subjects, TBI patients with cognitive impairment, and TBI patients without cognitive impairment were analysed to identify differentially expressed proteins (DEPs) related to post-TBI cognitive impairment.
In addition, DEPs were further analysed using bioinformatic platforms and validated using enzyme-linked immunosorbent assays (ELISA).
Results.
A total of 56 DEPs were identified that were specifically related to TBI-induced cognitive impairment.
Bioinformatic analysis revealed that a wide variety of cellular and metabolic processes and some signaling pathways were involved in the pathophysiology of cognitive deficits following TBI.
Five randomly selected DEPs were validated using ELISA in an additional 105 cases, and the results also supported the experimental findings.
Conclusions.
Despite limitations, our findings will facilitate further studies of the pathological mechanisms underlying TBI-induced cognitive impairment and provide new methods for the research and development of neuroprotective agents.
However, further investigation on a large cohort is warranted.
American Psychological Association (APA)
Xiong, Xin-Gui& Liang, Qinghua& Zhang, Chunhu& Wang, Yang& Huang, Wei& Peng, Weijun…[et al.]. 2017. Serum Proteome Alterations in Patients with Cognitive Impairment after Traumatic Brain Injury Revealed by iTRAQ-Based Quantitative Proteomics. BioMed Research International،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1139157
Modern Language Association (MLA)
Xiong, Xin-Gui…[et al.]. Serum Proteome Alterations in Patients with Cognitive Impairment after Traumatic Brain Injury Revealed by iTRAQ-Based Quantitative Proteomics. BioMed Research International No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1139157
American Medical Association (AMA)
Xiong, Xin-Gui& Liang, Qinghua& Zhang, Chunhu& Wang, Yang& Huang, Wei& Peng, Weijun…[et al.]. Serum Proteome Alterations in Patients with Cognitive Impairment after Traumatic Brain Injury Revealed by iTRAQ-Based Quantitative Proteomics. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1139157
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1139157