Blood and Bronchoalveolar Lavage Fluid Metagenomic Next-Generation Sequencing in Pneumonia
Joint Authors
Chen, Xu
Ding, Shuizi
Lei, Cheng
Qin, Jieli
Guo, Ting
Yang, Danhui
Yang, Min
Qing, Jie
He, Wenlong
Song, Min
Zhang, Yan
Zeng, Huihui
Qin, Qingwu
Yang, Lizhen
Long, Yingjiao
Chen, Yan
Ma, Bingyin
Ouyang, Ruoyun
Chen, Ping
Luo, Hong
Source
Canadian Journal of Infectious Diseases and Medical Microbiology
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-08-17
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background.
Metagenomic next-generation sequencing (mNGS) has made a revolution in the mode of pathogen identification.
We decided to explore the diagnostic value of blood and bronchoalveolar lavage fluid (BALF) as mNGS samples in pneumonia.
Methods.
We retrospectively reviewed 467 mNGS results and assessed the diagnostic performance of paired blood and BALF mNGS in 39 patients with pneumonia.
Results.
For bacteria and fungi, 16 patients had culture-confirmed pathogen diagnosis, while 13 patients were culture-negative.
BALF mNGS was more sensitive than blood mNGS (81.3% vs.
25.0%, p=0.003), and the specificity in BALF and blood mNGS was not statistically significant different (76.9% vs.
84.6%, p=0.317).
For 10 patients without culture test, treatments were changed in 2 patients.
For viruses, Epstein-Barr virus was positive in blood mNGS in 9 patients.
Human adenovirus was detected in both BALF and blood mNGS in 3 patients.
Conclusion.
Our study suggests that BALF mNGS is more sensitive than blood mNGS in detecting bacteria and fungi, but blood also has advantages to identify the pathogens of pneumonia, especially for some viruses.
American Psychological Association (APA)
Chen, Xu& Ding, Shuizi& Lei, Cheng& Qin, Jieli& Guo, Ting& Yang, Danhui…[et al.]. 2020. Blood and Bronchoalveolar Lavage Fluid Metagenomic Next-Generation Sequencing in Pneumonia. Canadian Journal of Infectious Diseases and Medical Microbiology،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1139161
Modern Language Association (MLA)
Chen, Xu…[et al.]. Blood and Bronchoalveolar Lavage Fluid Metagenomic Next-Generation Sequencing in Pneumonia. Canadian Journal of Infectious Diseases and Medical Microbiology No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1139161
American Medical Association (AMA)
Chen, Xu& Ding, Shuizi& Lei, Cheng& Qin, Jieli& Guo, Ting& Yang, Danhui…[et al.]. Blood and Bronchoalveolar Lavage Fluid Metagenomic Next-Generation Sequencing in Pneumonia. Canadian Journal of Infectious Diseases and Medical Microbiology. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1139161
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1139161