Combination of Neuroprotective and Regenerative Agents for AGE-Induced Retinal Degeneration: In Vitro Study
Joint Authors
Bikbova, Guzel
Oshitari, Toshiyuki
Baba, Takayuki
Yamamoto, Shuichi
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-05-09
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
To determine the most effective combination of neuroprotective and regenerative agents for cultured retinal neurons from advanced glycation end products- (AGEs-) induced degeneration, retinal explants of 7 adult Sprague-Dawley rats were three-dimensionally cultured in collagen gel and incubated in serum-free media and in 7 media; namely, AGEs, AGEs + 100 μM citicoline, AGEs + 10 ng/mL NT-4, AGEs + 100 μM TUDCA, AGEs + 100 μM citicoline + TUDCA (doublet), and AGEs + 100 μM citicoline + TUDCA + 10 ng/mL NT-4 (triplet) were examined.
The number of regenerating neurites was counted after 7 days of culture, followed by performing TUNEL and DAPI staining.
The ratio of TUNEL-positive cells to the number of DAPI-stained nuclei was calculated.
Immunohistochemical examinations for the active form of caspase-9 and JNK were performed.
All of the neuroprotectants increased the number of neurites and decreased the number of TUNEL-positive cells.
However, the number of neurites was significantly higher, and the number of TUNEL-positive cells and caspase-9- and JNK-immunopositive cells was fewer in the retinas incubated with the combined three agents.
Combination solutions containing citicoline, TUDCA, and NT-4 should be considered for neuroprotective and regenerative therapy for AGE-related retinal degeneration.
American Psychological Association (APA)
Bikbova, Guzel& Oshitari, Toshiyuki& Baba, Takayuki& Yamamoto, Shuichi. 2017. Combination of Neuroprotective and Regenerative Agents for AGE-Induced Retinal Degeneration: In Vitro Study. BioMed Research International،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1139180
Modern Language Association (MLA)
Bikbova, Guzel…[et al.]. Combination of Neuroprotective and Regenerative Agents for AGE-Induced Retinal Degeneration: In Vitro Study. BioMed Research International No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1139180
American Medical Association (AMA)
Bikbova, Guzel& Oshitari, Toshiyuki& Baba, Takayuki& Yamamoto, Shuichi. Combination of Neuroprotective and Regenerative Agents for AGE-Induced Retinal Degeneration: In Vitro Study. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1139180
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1139180