Osteogenic Differentiation Capacity of In Vitro Cultured Human Skeletal Muscle for Expedited Bone Tissue Engineering
Joint Authors
Liu, Fangjun
Miao, Chunlei
Zhou, Lulu
Tian, Lufeng
Zhang, Yingjie
Zhang, Wei
Yang, Fanghong
Liu, Tianyi
Tang, Shengjian
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-22
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Expedited bone tissue engineering employs the biological stimuli to harness the intrinsic regenerative potential of skeletal muscle to trigger the reparative process in situ to improve or replace biological functions.
When genetically modified with adenovirus mediated BMP2 gene transfer, muscle biopsies from animals have demonstrated success in regenerating bone within rat bony defects.
However, it is uncertain whether the human adult skeletal muscle displays an osteogenic potential in vitro when a suitable biological trigger is applied.
In present study, human skeletal muscle cultured in a standard osteogenic medium supplemented with dexamethasone demonstrated significant increase in alkaline phosphatase activity approximately 24-fold over control at 2-week time point.
More interestingly, measurement of mRNA levels revealed the dramatic results for osteoblast transcripts of alkaline phosphatase, bone sialoproteins, transcription factor CBFA1, collagen type I, and osteocalcin.
Calcified mineral deposits were demonstrated on superficial layers of muscle discs after an extended 8-week osteogenic induction.
Taken together, these are the first data supporting human skeletal muscle tissue as a promising potential target for expedited bone regeneration, which of the technologies is a valuable method for tissue repair, being not only effective but also inexpensive and clinically expeditious.
American Psychological Association (APA)
Miao, Chunlei& Zhou, Lulu& Tian, Lufeng& Zhang, Yingjie& Zhang, Wei& Yang, Fanghong…[et al.]. 2017. Osteogenic Differentiation Capacity of In Vitro Cultured Human Skeletal Muscle for Expedited Bone Tissue Engineering. BioMed Research International،Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1139194
Modern Language Association (MLA)
Miao, Chunlei…[et al.]. Osteogenic Differentiation Capacity of In Vitro Cultured Human Skeletal Muscle for Expedited Bone Tissue Engineering. BioMed Research International No. 2017 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-1139194
American Medical Association (AMA)
Miao, Chunlei& Zhou, Lulu& Tian, Lufeng& Zhang, Yingjie& Zhang, Wei& Yang, Fanghong…[et al.]. Osteogenic Differentiation Capacity of In Vitro Cultured Human Skeletal Muscle for Expedited Bone Tissue Engineering. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1139194
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1139194