Multiplexed 129Xe HyperCEST MRI Detection of Genetically Reconstituted Bacterial Protein Nanoparticles in Human Cancer Cells
Joint Authors
Mizushima, Ryota
Inoue, Kanako
Fujiwara, Hideaki
Iwane, Atsuko H.
Watanabe, Tomonobu M.
Kimura, Atsuomi
Source
Contrast Media & Molecular Imaging
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-03-12
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Gas vesicle nanoparticles (GVs) are gas-containing protein assemblies expressed in bacteria and archaea.
Recently, GVs have gained considerable attention for biotechnological applications as genetically encodable contrast agents for MRI and ultrasonography.
However, at present, the practical use of GVs is hampered by a lack of robust methodology for their induction into mammalian cells.
Here, we demonstrate the genetic reconstitution of protein nanoparticles with characteristic bicone structures similar to natural GVs in a human breast cancer cell line KPL-4 and genetic control of their size and shape through expression of reduced sets of humanized gas vesicle genes cloned into Tol2 transposon vectors, referencing the natural gas vesicle gene clusters of the cyanobacteria planktothrix rubescens/agardhii.
We then report the utility of these nanoparticles as multiplexed, sensitive, and genetically encoded contrast agents for hyperpolarized xenon chemical exchange saturation transfer (HyperCEST) MRI.
American Psychological Association (APA)
Mizushima, Ryota& Inoue, Kanako& Fujiwara, Hideaki& Iwane, Atsuko H.& Watanabe, Tomonobu M.& Kimura, Atsuomi. 2020. Multiplexed 129Xe HyperCEST MRI Detection of Genetically Reconstituted Bacterial Protein Nanoparticles in Human Cancer Cells. Contrast Media & Molecular Imaging،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1139265
Modern Language Association (MLA)
Mizushima, Ryota…[et al.]. Multiplexed 129Xe HyperCEST MRI Detection of Genetically Reconstituted Bacterial Protein Nanoparticles in Human Cancer Cells. Contrast Media & Molecular Imaging No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1139265
American Medical Association (AMA)
Mizushima, Ryota& Inoue, Kanako& Fujiwara, Hideaki& Iwane, Atsuko H.& Watanabe, Tomonobu M.& Kimura, Atsuomi. Multiplexed 129Xe HyperCEST MRI Detection of Genetically Reconstituted Bacterial Protein Nanoparticles in Human Cancer Cells. Contrast Media & Molecular Imaging. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1139265
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1139265