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SHISA3 Promoter Methylation Is a Potential Diagnostic and Prognostic Biomarker for Laryngeal Squamous Cell Carcinoma
Joint Authors
Shen, Zhisen
Zhou, Chongchang
Li, Jinyun
Ye, Dong
Deng, Hongxia
Cao, Bin
Hao, Wenjuan
Lin, Lexi
Zhang, Yuna
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-02-16
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The purpose of this study was to evaluate the contribution of SHISA3 promoter methylation to laryngeal squamous cell carcinoma (LSCC).
SHISA3 promoter methylation status and expression were determined using methylation-specific polymerase chain reaction (MSP) and quantitative real-time PCR (qRT-PCR) in 93 paired LSCC and adjacent normal tissues, respectively.
Furthermore, the regulatory function of the SHISA3 promoter fragment was analyzed using a luciferase reporter assay.
The results reveal that there is a significant increase in SHISA3 methylation in LSCC tissues compared with corresponding nontumor tissues P=4.58E-12.
The qRT-PCR results show a significant association between SHISA3 methylation and expression in LSCC P=1.67E-03.
In addition, the area under the receiver operating characteristic curve was 0.91.
Consequently, a log-rank test and multivariate Cox analysis suggest that SHISA3 promoter hypermethylation is a predictor of poor overall survival for LSCC (log-rank P = 0.024; HR = 2.71; 95% CI = 1.024–7.177; P = 0.047).
The results indicate that SHISA3 promoter hypermethylation might increase the risk of LSCC through regulation of gene expression and is a potential diagnostic and prognostic biomarker for LSCC.
American Psychological Association (APA)
Shen, Zhisen& Zhou, Chongchang& Li, Jinyun& Ye, Dong& Deng, Hongxia& Cao, Bin…[et al.]. 2017. SHISA3 Promoter Methylation Is a Potential Diagnostic and Prognostic Biomarker for Laryngeal Squamous Cell Carcinoma. BioMed Research International،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1139316
Modern Language Association (MLA)
Shen, Zhisen…[et al.]. SHISA3 Promoter Methylation Is a Potential Diagnostic and Prognostic Biomarker for Laryngeal Squamous Cell Carcinoma. BioMed Research International No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1139316
American Medical Association (AMA)
Shen, Zhisen& Zhou, Chongchang& Li, Jinyun& Ye, Dong& Deng, Hongxia& Cao, Bin…[et al.]. SHISA3 Promoter Methylation Is a Potential Diagnostic and Prognostic Biomarker for Laryngeal Squamous Cell Carcinoma. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1139316
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1139316