Exploring the Association of Surface Plasmon Resonance with Recombinant MHC:Ig Hybrid Protein as a Tool for Detecting T Lymphocytes in Mice Infected with Leishmania (Leishmania) amazonensis
Joint Authors
Alves, Carlos R.
Silveira-Júnior, Lenilton Silva da
Souza-Silva, Franklin
Pereira, Bernardo Acácio Santini
Cysne-Finkelstein, Léa
Cavalcanti Júnior, Geraldo Barroso
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-03-08
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
A surface plasmon resonance- (SPR-) based recognition method applying H-2 Ld:Ig/peptides complexes for ex vivo monitoring cellular immune responses during murine infection with Leishmania (Leishmania) amazonensis is described.
Lymphocytes from lesion-draining popliteal lymph nodes were captured on a carboxylated sensor chip surface previously functionalized with H-2 Ld:Ig (DimerX) protein bound to synthetic peptides derived from the COOH-terminal region of cysteine proteinase B of L.
(L.) amazonensis.
In computational analysis, these peptides presented values of kinetic constants favorable to form complexes with H-2 Ld at neutral pH, with a Gibbs free energy ΔG°<0.
The assayed DimerX:peptide complexes presented the property of attaching to distinct T lymphocytes subsets, obtained from experimentally infected BALB/c mice, in each week of infection, thus indicating a temporal variation in specific T lymphocytes populations, each directed to a different COOH-terminal region-derived peptide.
The experimental design proposed herein is an innovative approach for cellular immunology studies of a neglected disease, providing a useful tool for the analysis of specific T lymphocytes subsets.
American Psychological Association (APA)
Silveira-Júnior, Lenilton Silva da& Souza-Silva, Franklin& Pereira, Bernardo Acácio Santini& Cysne-Finkelstein, Léa& Cavalcanti Júnior, Geraldo Barroso& Alves, Carlos R.. 2017. Exploring the Association of Surface Plasmon Resonance with Recombinant MHC:Ig Hybrid Protein as a Tool for Detecting T Lymphocytes in Mice Infected with Leishmania (Leishmania) amazonensis. BioMed Research International،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1139341
Modern Language Association (MLA)
Silveira-Júnior, Lenilton Silva da…[et al.]. Exploring the Association of Surface Plasmon Resonance with Recombinant MHC:Ig Hybrid Protein as a Tool for Detecting T Lymphocytes in Mice Infected with Leishmania (Leishmania) amazonensis. BioMed Research International No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1139341
American Medical Association (AMA)
Silveira-Júnior, Lenilton Silva da& Souza-Silva, Franklin& Pereira, Bernardo Acácio Santini& Cysne-Finkelstein, Léa& Cavalcanti Júnior, Geraldo Barroso& Alves, Carlos R.. Exploring the Association of Surface Plasmon Resonance with Recombinant MHC:Ig Hybrid Protein as a Tool for Detecting T Lymphocytes in Mice Infected with Leishmania (Leishmania) amazonensis. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1139341
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1139341