miR-139-5p Inhibits Lung Adenocarcinoma Cell Proliferation, Migration, and Invasion by Targeting MAD2L1

Abstract EN

Background.

miR-139-5p is lowly expressed in various human cancers and exerts its antitumor effect through different molecular mechanisms, yet the molecular mechanism of miR-139-5p in lung adenocarcinoma (LUAD) remains to be further elucidated.

The study is aimed at investigating the role and the regulatory mechanism of miR-139-5p in LUAD progression.

Methods.

Differential analysis was performed on miRNA expression data in the TCGA-LUAD dataset.

qRT-PCR was employed to detect the transcription levels of miR-139-5p and MAD2L1 in LUAD cells, while western blot was carried out for the detection of MAD2L1 protein expression.

CCK-8 and Transwell assays were implemented to assess LUAD cell proliferation, migration, and invasion.

A dual-luciferase reporter gene assay was conducted to verify the direct targeting relationship between miR-139-5p and MAD2L1.

Results.

miR-139-5p was significantly downregulated in LUAD cells in comparison with that in human normal bronchial epithelial cells.

Overexpressing miR-139-5p inhibited LUAD cell proliferation, migration, and invasion, while opposite results could be observed when miR-139-5p was inhibited.

MAD2L1 was identified as a direct target of miR-139-5p in LUAD.

Besides, the inhibitory effect of miR-139-5p overexpression on LUAD cell proliferation, migration, and invasion was attenuated by overexpressing MAD2L1.

Conclusion.

Our study suggests that miR-139-5p is lowly expressed in LUAD cells and inhibits LUAD cell proliferation, migration, and invasion by targeted suppressing MAD2L1 expression.

It is of potential significance for the prognosis and treatment of LUAD.