In Silico Analysis Identifies Differently Expressed lncRNAs as Novel Biomarkers for the Prognosis of Thyroid Cancer

Abstract EN

Background.

Thyroid cancer (TC) is one of the most common type of endocrine tumors.

Long noncoding RNAs had been demonstrated to play key roles in TC.

Material and Methods.

The lncRNA expression data were downloaded from Co-lncRNA database.

The raw data was normalized using the limma package in R software version 3.3.0.

The differentially expressed mRNA and lncRNAs were identified by the linear models for the microarray analysis (Limma) method.

The DEGs were obtained with thresholds of ∣logFC∣>1.5 and P<0.001.

The hierarchical cluster analysis of differentially expressed mRNAs and lncRNAs was performed using CLUSTER 3.0, and the hierarchical clustering heat map was visualized by Tree View.

Results.

In the present study, we identified 6 upregulated and 85 downregulated lncRNAs in TC samples.

Moreover, we for the first time identified 16 downregulated lncRNAs was correlated to longer disease-free survival time in patients with TC, including ATP1A1-AS1, CATIP-AS1, FAM13A-AS1, LINC00641, LINC00924, MIR22HG, NDUFA6-AS1, RP11-175K6.1, RP11-727A23.5, RP11-774O3.3, RP13-895J2.2, SDCBP2-AS1, SLC26A4-AS1, SNHG15, SRP14-AS1, and ZNF674-AS1.

Conclusions.

Bioinformatics analysis revealed these lncRNAs were involved in regulating the RNA metabolic process, cell migration, organelle assembly, tRNA modification, and hormone levels.

This study will provide useful information to explore the potential candidate biomarkers for diagnosis, prognosis, and drug targets for TC.