Current Nucleic Acid Extraction Methods and Their Implications to Point-of-Care Diagnostics
Joint Authors
de Cássia Pontello Rampazzo, Rita
Krieger, Marco A.
Ali, Nasir
Costa, Alexandre Dias Tavares
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-12
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Nucleic acid extraction (NAE) plays a vital role in molecular biology as the primary step for many downstream applications.
Many modifications have been introduced to the original 1869 method.
Modern processes are categorized into chemical or mechanical, each with peculiarities that influence their use, especially in point-of-care diagnostics (POC-Dx).
POC-Dx is a new approach aiming to replace sophisticated analytical machinery with microanalytical systems, able to be used near the patient, at the point of care or point of need.
Although notable efforts have been made, a simple and effective extraction method is still a major challenge for widespread use of POC-Dx.
In this review, we dissected the working principle of each of the most common NAE methods, overviewing their advantages and disadvantages, as well their potential for integration in POC-Dx systems.
At present, it seems difficult, if not impossible, to establish a procedure which can be universally applied to POC-Dx.
We also discuss the effects of the NAE chemicals upon the main plastic polymers used to mass produce POC-Dx systems.
We end our review discussing the limitations and challenges that should guide the quest for an efficient extraction method that can be integrated in a POC-Dx system.
American Psychological Association (APA)
Ali, Nasir& de Cássia Pontello Rampazzo, Rita& Costa, Alexandre Dias Tavares& Krieger, Marco A.. 2017. Current Nucleic Acid Extraction Methods and Their Implications to Point-of-Care Diagnostics. BioMed Research International،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1139456
Modern Language Association (MLA)
Ali, Nasir…[et al.]. Current Nucleic Acid Extraction Methods and Their Implications to Point-of-Care Diagnostics. BioMed Research International No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1139456
American Medical Association (AMA)
Ali, Nasir& de Cássia Pontello Rampazzo, Rita& Costa, Alexandre Dias Tavares& Krieger, Marco A.. Current Nucleic Acid Extraction Methods and Their Implications to Point-of-Care Diagnostics. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1139456
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1139456