Efficacy of Eucalyptus cinerea as a Source of Bioactive Compounds for Curative Biocontrol of Crown Gall Caused by Agrobacterium tumefaciens Strain B6
Joint Authors
Tounsi, S.
Zouari Bouassida, Karama
Trigui, Mohamed
Kahla, Yosra
Rezgui, Fatma
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-03
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
This research investigated the Eucalyptus cinerea leaves efficiency in the Agrobacterium tumefaciens biocontrol, the causative agent of crown gall.
GC-MS analysis of the essential oil (EO) showed that the main components were 1,8-cineole (61%) and camphene (15.13%).
Thanks to its polyphenols, flavonoids, quinones, terpenoids, alkaloids, and tannins richness, the EtOAc-F exhibited the most potent antibacterial activity in vitro.
Indeed, compared to the other fractions, it has the lowest MIC and MBC values of 0.312 mg/mL and 2.5 mg/mL, respectively.
The GC-MS analysis of EtOAc-F confirmed its richness in antibacterial compounds including gallic acid (7.18%), shikimic acid (5.07%), and catechin (3.12%).
The time-kill curve assay of EtOAc-F (2.5 mg/mL) showed a potent bactericidal effect after 20 min of direct contact with A.
tumefaciens.
In planta experiments, gall weights were significantly reduced when EtOAc-F was applied at 0.625 and 2.5 mg/wounds.
Besides, the disease reduction rates in gall weight were 95% and 97.5%, respectively.
Interestingly, no phytotoxic effect was observed since tomato seeds germinated in the presence of the different concentrations of EtOAc-F.
These results suggest that EtOAc-F has a good potential to be a curative biocontrol agent for crown gall disease.
American Psychological Association (APA)
Kahla, Yosra& Zouari Bouassida, Karama& Rezgui, Fatma& Trigui, Mohamed& Tounsi, S.. 2017. Efficacy of Eucalyptus cinerea as a Source of Bioactive Compounds for Curative Biocontrol of Crown Gall Caused by Agrobacterium tumefaciens Strain B6. BioMed Research International،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1139458
Modern Language Association (MLA)
Kahla, Yosra…[et al.]. Efficacy of Eucalyptus cinerea as a Source of Bioactive Compounds for Curative Biocontrol of Crown Gall Caused by Agrobacterium tumefaciens Strain B6. BioMed Research International No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1139458
American Medical Association (AMA)
Kahla, Yosra& Zouari Bouassida, Karama& Rezgui, Fatma& Trigui, Mohamed& Tounsi, S.. Efficacy of Eucalyptus cinerea as a Source of Bioactive Compounds for Curative Biocontrol of Crown Gall Caused by Agrobacterium tumefaciens Strain B6. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1139458
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1139458