Enhancement of Angiogenesis by Ultrasound-Targeted Microbubble Destruction Combined with Nuclear Localization Signaling Peptides in Canine Myocardial Infarction

Joint Authors

Cui, Jingjing
Deng, Qing
Zhou, Qing
Cao, Sheng
Jiang, Nan
Wang, Yijia
Chen, Jinling
Hu, Bo
Tan, Tuantuan

Source

BioMed Research International

Issue

Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2017-11-12

Country of Publication

Egypt

No. of Pages

10

Main Subjects

Medicine

Abstract EN

Objective.

This study aimed to develop a gene delivery system using ultrasound-targeted microbubbles destruction (UTMD) combined with nuclear localization signal (NLS) and investigate its efficacy and safety for therapeutic angiogenesis in canine myocardial infarction (MI) model.

Methods.

Fifty MI dogs were randomly divided into 5 groups and transfected with Ang-1 gene plasmid: (i) group A: only injection of microbubbles and Ang-1 plasmid; (ii) group B: only UTMD mediated gene transfection; (iii) group C: UTMD combined with classical NLS mediated gene transfection; (iv) group D: UTMD combined with mutational NLS mediated transfection; and (v) group E: UTMD combined with classical NLS in the presence of a nucleus transport blocker.

The mRNA and protein expression of Ang-1 gene, microvessel density (MVD) cardiac troponin I (cTnI), and cardiac function were determined after transfection.

Results.

The expression of mRNA and protein of Ang-1 gene in group C was significantly higher than that of the other groups (all P < 0.01).

The MVD of group C was 10.2-fold of group A and 8.1-fold of group E (P < 0.01).

The cardiac function in group C was significant improvement without cTnI rising.

Conclusions.

The gene delivery system composed of UTMD and NLS is efficient and safe.

American Psychological Association (APA)

Cui, Jingjing& Deng, Qing& Zhou, Qing& Cao, Sheng& Jiang, Nan& Wang, Yijia…[et al.]. 2017. Enhancement of Angiogenesis by Ultrasound-Targeted Microbubble Destruction Combined with Nuclear Localization Signaling Peptides in Canine Myocardial Infarction. BioMed Research International،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1139513

Modern Language Association (MLA)

Cui, Jingjing…[et al.]. Enhancement of Angiogenesis by Ultrasound-Targeted Microbubble Destruction Combined with Nuclear Localization Signaling Peptides in Canine Myocardial Infarction. BioMed Research International No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1139513

American Medical Association (AMA)

Cui, Jingjing& Deng, Qing& Zhou, Qing& Cao, Sheng& Jiang, Nan& Wang, Yijia…[et al.]. Enhancement of Angiogenesis by Ultrasound-Targeted Microbubble Destruction Combined with Nuclear Localization Signaling Peptides in Canine Myocardial Infarction. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1139513

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1139513