Laminin-521 Promotes Rat Bone Marrow Mesenchymal Stem Cell Sheet Formation on Light-Induced Cell Sheet Technology

Abstract EN

Rat bone marrow mesenchymal stem cell sheets (rBMSC sheets) are attractive for cell-based tissue engineering.

However, methods of culturing rBMSC sheets are critically limited.

In order to obtain intact rBMSC sheets, a light-induced cell sheet method was used in this study.

TiO2 nanodot films were coated with (TL) or without (TN) laminin-521.

We investigated the effects of laminin-521 on rBMSCs during cell sheet culturing.

The fabricated rBMSC sheets were subsequently assessed to study cell sheet viability, reattachment ability, cell sheet thickness, collagen type I deposition, and multilineage potential.

The results showed that laminin-521 could promote the formation of rBMSC sheets with good viability under hyperconfluent conditions.

Cell sheet thickness increased from an initial 26.7 ± 1.5 μm (day 5) up to 47.7 ± 3.0 μm (day 10).

Moreover, rBMSC sheets maintained their potential of osteogenic, adipogenic, and chondrogenic differentiation.

This study provides a new strategy to obtain rBMSC sheets using light-induced cell sheet technology.