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Laminin-521 Promotes Rat Bone Marrow Mesenchymal Stem Cell Sheet Formation on Light-Induced Cell Sheet Technology
Joint Authors
Yang, Guoli
Jiang, Zhiwei
Xi, Yue
Lai, Kaichen
Wang, Ying
Wang, Huiming
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-09
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Rat bone marrow mesenchymal stem cell sheets (rBMSC sheets) are attractive for cell-based tissue engineering.
However, methods of culturing rBMSC sheets are critically limited.
In order to obtain intact rBMSC sheets, a light-induced cell sheet method was used in this study.
TiO2 nanodot films were coated with (TL) or without (TN) laminin-521.
We investigated the effects of laminin-521 on rBMSCs during cell sheet culturing.
The fabricated rBMSC sheets were subsequently assessed to study cell sheet viability, reattachment ability, cell sheet thickness, collagen type I deposition, and multilineage potential.
The results showed that laminin-521 could promote the formation of rBMSC sheets with good viability under hyperconfluent conditions.
Cell sheet thickness increased from an initial 26.7 ± 1.5 μm (day 5) up to 47.7 ± 3.0 μm (day 10).
Moreover, rBMSC sheets maintained their potential of osteogenic, adipogenic, and chondrogenic differentiation.
This study provides a new strategy to obtain rBMSC sheets using light-induced cell sheet technology.
American Psychological Association (APA)
Jiang, Zhiwei& Xi, Yue& Lai, Kaichen& Wang, Ying& Wang, Huiming& Yang, Guoli. 2017. Laminin-521 Promotes Rat Bone Marrow Mesenchymal Stem Cell Sheet Formation on Light-Induced Cell Sheet Technology. BioMed Research International،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1139550
Modern Language Association (MLA)
Jiang, Zhiwei…[et al.]. Laminin-521 Promotes Rat Bone Marrow Mesenchymal Stem Cell Sheet Formation on Light-Induced Cell Sheet Technology. BioMed Research International No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1139550
American Medical Association (AMA)
Jiang, Zhiwei& Xi, Yue& Lai, Kaichen& Wang, Ying& Wang, Huiming& Yang, Guoli. Laminin-521 Promotes Rat Bone Marrow Mesenchymal Stem Cell Sheet Formation on Light-Induced Cell Sheet Technology. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1139550
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1139550