Long Noncoding RNA HAGLROS Promotes Cell Invasion and Metastasis by Sponging miR-152 and Upregulating ROCK1 Expression in Osteosarcoma
Joint Authors
Xu, Jun
Zhou, Kaifeng
Yin, Xiaofan
Xia, Jiangni
Source
Computational and Mathematical Methods in Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-09-09
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background.
Long noncoding RNAs (lncRNAs) played a crucial role in a number of biological processes.
lncRNA HAGLROS was demonstrated to facilitate cell proliferation and migration in various cancers.
However, the functions and molecular mechanisms of HAGLROS in osteosarcoma remained to be elucidated.
Methods.
qRT-PCR assay was used to detect the relative expression of HAGLROS in osteosarcoma tissue samples and cells.
CCK-8 and Transwell assays were performed to assess the effects of HAGLROS on OS cells proliferation and invasion.
Luciferase reporter assay verified the interaction between ROCK1 and miR-152.
Results.
In our study, we found that the expression of HAGLROS increased osteosarcoma samples and cell lines compared with normal tissues and cells.
HAGLROS knockdown inhibited certain functions of U2OS and SW1353 cells in vitro.
Moreover, HAGLROS depletion inhibited tumor growth and metastasis in vivo.
Mechanically, we found that HAGLROS sponged miR-152 to promote ROCK1 expression in U2OS and SW1353 cells.
Conclusion.
In summary, our study indicated that HAGLROS could promote osteosarcoma progression by sponging miR-152 to promote ROCK1 expression.
The results showed HAGLROS/miR-152/ROCK1 axis might act as a novel therapeutic strategy for osteosarcoma.
American Psychological Association (APA)
Zhou, Kaifeng& Xu, Jun& Yin, Xiaofan& Xia, Jiangni. 2020. Long Noncoding RNA HAGLROS Promotes Cell Invasion and Metastasis by Sponging miR-152 and Upregulating ROCK1 Expression in Osteosarcoma. Computational and Mathematical Methods in Medicine،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1139556
Modern Language Association (MLA)
Zhou, Kaifeng…[et al.]. Long Noncoding RNA HAGLROS Promotes Cell Invasion and Metastasis by Sponging miR-152 and Upregulating ROCK1 Expression in Osteosarcoma. Computational and Mathematical Methods in Medicine No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1139556
American Medical Association (AMA)
Zhou, Kaifeng& Xu, Jun& Yin, Xiaofan& Xia, Jiangni. Long Noncoding RNA HAGLROS Promotes Cell Invasion and Metastasis by Sponging miR-152 and Upregulating ROCK1 Expression in Osteosarcoma. Computational and Mathematical Methods in Medicine. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1139556
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1139556