Esculetin Neutralises Cytotoxicity of t-BHP but Not of H2O2 on Human Leukaemia NB4 Cells
Joint Authors
Rubio, Virginia
García-Pérez, Ana I.
Tejedor, M. Cristina
Herráez, Angel
Diez, José C.
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-03-07
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The coumarin esculetin shows antioxidant action on some cell types, both by scavenging ROS and by decreasing ROS production.
We have previously demonstrated the induction of apoptosis by esculetin on NB4 human leukaemia cells by an ill-defined mechanism related to ROS levels.
In this work, we analyze the effect of the simultaneous treatment with esculetin and two oxidants to observe the early events in the mechanism of esculetin-induced apoptosis.
Our results show that, from the early time of 15 min, esculetin acts synergistically with H2O2 to decrease cell viability and metabolic activity and to increase apoptosis in NB4 cells.
In contrast, the early oxidative effects of t-BHP are neutralised by esculetin, protecting human leukaemia NB4 cells from apoptosis.
Esculetin seems to restrict the increase in peroxides caused by H2O2 or t-BHP in the time interval analyzed.
These results contribute to a better understanding of the cytotoxic effect caused by esculetin on NB4 cells.
At the same time, the early neutralisation of exogenous oxidants could be of interest to prevent diseases related to oxidative stress imbalance.
American Psychological Association (APA)
Rubio, Virginia& García-Pérez, Ana I.& Tejedor, M. Cristina& Herráez, Angel& Diez, José C.. 2017. Esculetin Neutralises Cytotoxicity of t-BHP but Not of H2O2 on Human Leukaemia NB4 Cells. BioMed Research International،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1139563
Modern Language Association (MLA)
Rubio, Virginia…[et al.]. Esculetin Neutralises Cytotoxicity of t-BHP but Not of H2O2 on Human Leukaemia NB4 Cells. BioMed Research International No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1139563
American Medical Association (AMA)
Rubio, Virginia& García-Pérez, Ana I.& Tejedor, M. Cristina& Herráez, Angel& Diez, José C.. Esculetin Neutralises Cytotoxicity of t-BHP but Not of H2O2 on Human Leukaemia NB4 Cells. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1139563
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1139563