SOX6 Downregulation Induces γ-Globin in Human β-Thalassemia Major Erythroid Cells
Joint Authors
Liu, Rongrong
Li, Jing
Lai, Yongrong
Luo, Jun
Luo, Lin
Liu, Zhenfang
Zhao, Weihua
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-11-28
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Background.
Fetal hemoglobin (HbF; α2γ2) is a potent genetic modifier of the severity of β-thalassemia and sickle cell anemia.
Differences in the levels of HbF that persist into adulthood affect the severity of sickle cell disease and the β-thalassemia syndromes.
Sry type HMG box (SOX6) is a potent silencer of HbF.
Here, we reactivated γ-globin expression by downregulating SOX6 to alleviate anemia in the β-thalassemia patients.
Methods.
SOX6 was downregulated by lentiviral RNAi (RNA interference) in K562 cell line and an in vitro culture model of human erythropoiesis in which erythroblasts are derived from the normal donor mononuclear cells (MNC) or β-thalassemia major MNC.
The expression of γ-globin was analyzed by qPCR (quantitative real-time PCR) and WB (western blot).
Results.
Our data showed that downregulation of SOX6 induces γ-globin production in K562 cell line and human erythrocytes from normal donors and β-thalassemia major donors, without altering erythroid maturation.
Conclusions.
This is the first report on γ-globin induction by downregulation of SOX6 in human erythroblasts derived from β-thalassemia major.
American Psychological Association (APA)
Li, Jing& Lai, Yongrong& Luo, Jun& Luo, Lin& Liu, Rongrong& Liu, Zhenfang…[et al.]. 2017. SOX6 Downregulation Induces γ-Globin in Human β-Thalassemia Major Erythroid Cells. BioMed Research International،Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1139565
Modern Language Association (MLA)
Li, Jing…[et al.]. SOX6 Downregulation Induces γ-Globin in Human β-Thalassemia Major Erythroid Cells. BioMed Research International No. 2017 (2017), pp.1-6.
https://search.emarefa.net/detail/BIM-1139565
American Medical Association (AMA)
Li, Jing& Lai, Yongrong& Luo, Jun& Luo, Lin& Liu, Rongrong& Liu, Zhenfang…[et al.]. SOX6 Downregulation Induces γ-Globin in Human β-Thalassemia Major Erythroid Cells. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1139565
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1139565