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Cytoplasmic Localization of WT1 and Decrease of miRNA-16-1 in Nephrotic Syndrome
Joint Authors
Arellano-Rodríguez, Mariela
Rodríguez-Padilla, Cristina
Franco-Molina, Moisés Armides
Zapata-Benavides, Pablo
Rangel-Ochoa, Gloria Azucena
Bollain-y-Goytia, Juan José
Herrera-Esparza, Rafael
Avalos-Díaz, Esperanza
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-02-19
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Nephrotic syndrome (NS) is a glomerular disease that is defined by the leakage of protein into the urine and is associated with hypoalbuminemia, hyperlipidemia, and edema.
Steroid-resistant NS (SRNS) patients do not respond to treatment with corticosteroids and show decreased Wilms tumor 1 (WT1) expression in podocytes.
Downregulation of WT1 has been shown to be affected by certain microRNAs (miRNAs).
Twenty-one patients with idiopathic NS (68.75% were SSNS and 31.25% SRNS) and 10 healthy controls were enrolled in the study.
Podocyte number and WT1 location were determined by immunofluorescence, and the serum levels of miR-15a, miR-16-1, and miR-193a were quantified by RT-qPCR.
Low expression and delocalization of WT1 protein from the nucleus to the cytoplasm were found in kidney biopsies of patients with SRNS and both nuclear and cytoplasmic localization were found in steroid-sensitive NS (SSNS) patients.
In sera from NS patients, low expression levels of miR-15a and miR-16-1 were found compared with healthy controls, but only the miR-16-1 expression levels showed statistically significant decrease (p=0.019).
The miR-193a expression levels only slightly increased in NS patients.
We concluded that low expression and delocalization from the WT1 protein in NS patients contribute to loss of podocytes while modulation from WT1 protein is not associated with the miRNAs analyzed in sera from the patients.
American Psychological Association (APA)
Zapata-Benavides, Pablo& Arellano-Rodríguez, Mariela& Bollain-y-Goytia, Juan José& Franco-Molina, Moisés Armides& Rangel-Ochoa, Gloria Azucena& Avalos-Díaz, Esperanza…[et al.]. 2017. Cytoplasmic Localization of WT1 and Decrease of miRNA-16-1 in Nephrotic Syndrome. BioMed Research International،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1139586
Modern Language Association (MLA)
Zapata-Benavides, Pablo…[et al.]. Cytoplasmic Localization of WT1 and Decrease of miRNA-16-1 in Nephrotic Syndrome. BioMed Research International No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1139586
American Medical Association (AMA)
Zapata-Benavides, Pablo& Arellano-Rodríguez, Mariela& Bollain-y-Goytia, Juan José& Franco-Molina, Moisés Armides& Rangel-Ochoa, Gloria Azucena& Avalos-Díaz, Esperanza…[et al.]. Cytoplasmic Localization of WT1 and Decrease of miRNA-16-1 in Nephrotic Syndrome. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1139586
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1139586