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RNA-Binding Protein Dnd1 Promotes Breast Cancer Apoptosis by Stabilizing the Bim mRNA in a miR-221 Binding Site
Joint Authors
Cheng, Feng
Pan, Ying
Lu, Yi-Min
Zhu, Lei
Chen, Shuzheng
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-16
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
RNA-binding proteins (RBPs) and miRNAs are capable of controlling processes in normal development and cancer.
Both of them could determine RNA transcripts fate from synthesis to decay.
One such RBP, Dead end (Dnd1), is essential for regulating germ-cell viability and suppresses the germ-cell tumors development, yet how it exerts its functions in breast cancer has remained unresolved.
The level of Dnd1 was detected in 21 cancerous tissues paired with neighboring normal tissues by qRT-PCR.
We further annotated TCGA (The Cancer Genome Atlas) mRNA expression profiles and found that the expression of Dnd1 and Bim is positively correlated ( p = 0 .
04 ).
Patients with higher Dnd1 expression level had longer overall survival ( p = 0 .
0014 ) by KM Plotter tool.
Dnd1 knockdown in MCF-7 cells decreased Bim expression levels and inhibited apoptosis.
While knockdown of Dnd1 promoted the decay of Bim mRNA 3′UTR, the stability of Bim-5′UTR was not affected.
In addition, mutation of miR-221-binding site in Bim-3′UTR canceled the effect of Dnd1 on Bim mRNA.
Knockdown of Dnd1 in MCF-7 cells confirmed that Dnd1 antagonized miR-221-inhibitory effects on Bim expression.
Overall, our findings indicate that Dnd1 facilitates apoptosis by increasing the expression of Bim via its competitive combining with miR-221 in Bim-3′UTR.
The new function of Dnd1 may contribute to a vital role in breast cancer development.
American Psychological Association (APA)
Cheng, Feng& Pan, Ying& Lu, Yi-Min& Zhu, Lei& Chen, Shuzheng. 2017. RNA-Binding Protein Dnd1 Promotes Breast Cancer Apoptosis by Stabilizing the Bim mRNA in a miR-221 Binding Site. BioMed Research International،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1139632
Modern Language Association (MLA)
Cheng, Feng…[et al.]. RNA-Binding Protein Dnd1 Promotes Breast Cancer Apoptosis by Stabilizing the Bim mRNA in a miR-221 Binding Site. BioMed Research International No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1139632
American Medical Association (AMA)
Cheng, Feng& Pan, Ying& Lu, Yi-Min& Zhu, Lei& Chen, Shuzheng. RNA-Binding Protein Dnd1 Promotes Breast Cancer Apoptosis by Stabilizing the Bim mRNA in a miR-221 Binding Site. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1139632
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1139632