Increased Expression of Toll-Like Receptors 4, 5, and 9 in Small Bowel Mucosa from Patients with Irritable Bowel Syndrome
Joint Authors
Lindberg, Greger
Dlugosz, Aldona
Acevedo, Nathalie
Zakikhany, Katherina
D’Amato, Mauro
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-29
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The aim of our study was to compare patients with irritable bowel syndrome (IBS) and healthy controls regarding the expression of toll-like receptors 2, 4, 5, and 9 (TLR2, TLR4, TLR5, and TLR9), the primary mucosal receptors of bacterial components, in small and large bowel mucosa.
Methods.
We analysed biopsies from jejunum and sigmoid colon of 22 patients (17 females) with IBS aged 18–66 (median: 39) years and 14 healthy volunteers (12 females) aged 22–61 (median: 42) years.
Eight patients had constipation-predominant IBS (C-IBS), 7 had diarrhoea-predominant IBS (D-IBS), and 7 had IBS without predominance of constipation or diarrhoea.
We analysed mRNA levels for TLRs using quantitative PCR and distribution of TLRs in mucosa using immunohistochemistry.
Results.
We found increased mRNA expression of TLR4 (mean fold change 1.85 ± 0.31 versus 1.0 ± 0.20 ; p < 0.05 ), TLR5 ( 1.96 ± 0.36 versus 1.0 ± 0.20 ; p < 0.05 ) and TLR9 ( 2.00 ± 0.24 versus 1.0 ± 0.25 ; p < 0.01 ) but not of TLR2 in the small bowel mucosa from patients with IBS compared to the controls.
There was no significant difference in mRNA levels for TLRs in colon mucosa between patients and controls.
Conclusion.
Upregulation of TLR4, TLR5, and TLR9 suggests the involvement of bacteria or dysregulation of the immune response to commensal flora in small bowel mucosa in IBS patients.
American Psychological Association (APA)
Dlugosz, Aldona& Zakikhany, Katherina& Acevedo, Nathalie& D’Amato, Mauro& Lindberg, Greger. 2017. Increased Expression of Toll-Like Receptors 4, 5, and 9 in Small Bowel Mucosa from Patients with Irritable Bowel Syndrome. BioMed Research International،Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1139658
Modern Language Association (MLA)
Dlugosz, Aldona…[et al.]. Increased Expression of Toll-Like Receptors 4, 5, and 9 in Small Bowel Mucosa from Patients with Irritable Bowel Syndrome. BioMed Research International No. 2017 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-1139658
American Medical Association (AMA)
Dlugosz, Aldona& Zakikhany, Katherina& Acevedo, Nathalie& D’Amato, Mauro& Lindberg, Greger. Increased Expression of Toll-Like Receptors 4, 5, and 9 in Small Bowel Mucosa from Patients with Irritable Bowel Syndrome. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1139658
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1139658