Evaluation of Epigallocatechin-3-gallate Modified Collagen Membrane and Concerns on Schwann Cells
Joint Authors
Man, Yi
Qu, Yili
Chu, Chenyu
Deng, Jia
Cao, Cong
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-08-15
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Collagen is an essential component of the extracellular matrix (ECM) and is a suitable material for nerve repair during tissue remodeling for fracture repair.
Epigallocatechin-3-gallate (EGCG), an extract of green tea, shows various biological activities that are beneficial to nerve repair.
Here, we developed modified collagen containing different concentrations of EGCG (0.0064%, 0.064%, and 0.64%, resp.) to induce Schwann cell proliferation and differentiation.
Cell Counting Kit-8 test, live/dead assay, and SEM showed that collagen cross-linked by EGCG induced Schwann cell proliferation.
Real-time polymerase chain reaction, enzyme-linked immunosorbent assay, and Western blotting revealed that EGCG-modified collagen induced Schwann cell differentiation and downregulated reactive oxygen species (ROS) levels by downregulating the MAPK P38 signaling pathway.
Our results indicate that collagen cross-linked with an appropriate concentration of EGCG induces the proliferation and differentiation of Schwann cells.
The EGCG-modified collagen membrane may be applicable for nerve repair and guided tissue regeneration applications.
American Psychological Association (APA)
Chu, Chenyu& Deng, Jia& Cao, Cong& Man, Yi& Qu, Yili. 2017. Evaluation of Epigallocatechin-3-gallate Modified Collagen Membrane and Concerns on Schwann Cells. BioMed Research International،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1139671
Modern Language Association (MLA)
Chu, Chenyu…[et al.]. Evaluation of Epigallocatechin-3-gallate Modified Collagen Membrane and Concerns on Schwann Cells. BioMed Research International No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1139671
American Medical Association (AMA)
Chu, Chenyu& Deng, Jia& Cao, Cong& Man, Yi& Qu, Yili. Evaluation of Epigallocatechin-3-gallate Modified Collagen Membrane and Concerns on Schwann Cells. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1139671
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1139671