Gene Expression Profiling of Type 2 Diabetes Mellitus by Bioinformatics Analysis
Joint Authors
Zhu, Huijing
Zhu, Xin
Liu, Yuhong
Jiang, Fusong
Chen, Miao
Cheng, Lin
Cheng, Xingbo
Source
Computational and Mathematical Methods in Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-10-23
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Objective.
The aim of this study was to identify the candidate genes in type 2 diabetes mellitus (T2DM) and explore their potential mechanisms.
Methods.
The gene expression profile GSE26168 was downloaded from the Gene Expression Omnibus (GEO) database.
The online tool GEO2R was used to obtain differentially expressed genes (DEGs).
Gene Ontology (GO) term enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed by using Metascape for annotation, visualization, and comprehensive discovery.
The protein-protein interaction (PPI) network of DEGs was constructed by using Cytoscape software to find the candidate genes and key pathways.
Results.
A total of 981 DEGs were found in T2DM, including 301 upregulated genes and 680 downregulated genes.
GO analyses from Metascape revealed that DEGs were significantly enriched in cell differentiation, cell adhesion, intracellular signal transduction, and regulation of protein kinase activity.
KEGG pathway analysis revealed that DEGs were mainly enriched in the cAMP signaling pathway, Rap1 signaling pathway, regulation of lipolysis in adipocytes, PI3K-Akt signaling pathway, MAPK signaling pathway, and so on.
On the basis of the PPI network of the DEGs, the following 6 candidate genes were identified: PIK3R1, RAC1, GNG3, GNAI1, CDC42, and ITGB1.
Conclusion.
Our data provide a comprehensive bioinformatics analysis of genes, functions, and pathways, which may be related to the pathogenesis of T2DM.
American Psychological Association (APA)
Zhu, Huijing& Zhu, Xin& Liu, Yuhong& Jiang, Fusong& Chen, Miao& Cheng, Lin…[et al.]. 2020. Gene Expression Profiling of Type 2 Diabetes Mellitus by Bioinformatics Analysis. Computational and Mathematical Methods in Medicine،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1139677
Modern Language Association (MLA)
Zhu, Huijing…[et al.]. Gene Expression Profiling of Type 2 Diabetes Mellitus by Bioinformatics Analysis. Computational and Mathematical Methods in Medicine No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1139677
American Medical Association (AMA)
Zhu, Huijing& Zhu, Xin& Liu, Yuhong& Jiang, Fusong& Chen, Miao& Cheng, Lin…[et al.]. Gene Expression Profiling of Type 2 Diabetes Mellitus by Bioinformatics Analysis. Computational and Mathematical Methods in Medicine. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1139677
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1139677