![](/images/graphics-bg.png)
Time- and Dose-Dependent Effects of 17 Beta-Estradiol on Short-Term, Real-Time Proliferation and Gene Expression in Porcine Granulosa Cells
Joint Authors
Antosik, Paweł
Nowicki, Michał
Zabel, Maciej
Kempisty, Bartosz
Ziolkowska, Agnieszka
Jopek, Karol
Ciesiółka, Sylwia
Budna, Joanna
Bryja, Artur
Kranc, Wiesława
Chachuła, Adrian
Borys, Sylwia
Bukowska, Dorota
Brüssow, Klaus P.
Bruska, Małgorzata
Jeseta, Michal
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-02-27
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The key mechanisms responsible for achievement of full reproductive and developmental capability in mammals are the differentiation and transformation of granulosa cells (GCs) during folliculogenesis, oogenesis, and oocyte maturation.
Although the role of 17 beta-estradiol (E2) in ovarian activity is widely known, its effect on proliferative capacity, gap junction connection (GJC) formation, and GCs-luteal cells transformation requires further research.
Therefore, the goal of this study was to assess the real-time proliferative activity of porcine GCs in vitro in relation to connexin (Cx), luteinizing hormone receptor (LHR), follicle stimulating hormone receptor (FSHR), and aromatase (CYP19A1) expression during short-term (168 h) primary culture.
The cultured GCs were exposed to acute (at 96 h of culture) and/or prolonged (between 0 and 168 h of culture) administration of 1.8 and 3.6 μM E2.
The relative abundance of Cx36, Cx37, Cx40, Cx43, LHR, FSHR, and CYP19A1 mRNA was measured.
We conclude that the proliferation capability of GCs in vitro is substantially associated with expression of Cxs, LHR, FSHR, and CYP19A1.
Furthermore, the GC-luteal cell transformation in vitro may be significantly accompanied by the proliferative activity of GCs in pigs.
American Psychological Association (APA)
Ciesiółka, Sylwia& Budna, Joanna& Jopek, Karol& Bryja, Artur& Kranc, Wiesława& Borys, Sylwia…[et al.]. 2017. Time- and Dose-Dependent Effects of 17 Beta-Estradiol on Short-Term, Real-Time Proliferation and Gene Expression in Porcine Granulosa Cells. BioMed Research International،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1139712
Modern Language Association (MLA)
Ciesiółka, Sylwia…[et al.]. Time- and Dose-Dependent Effects of 17 Beta-Estradiol on Short-Term, Real-Time Proliferation and Gene Expression in Porcine Granulosa Cells. BioMed Research International No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1139712
American Medical Association (AMA)
Ciesiółka, Sylwia& Budna, Joanna& Jopek, Karol& Bryja, Artur& Kranc, Wiesława& Borys, Sylwia…[et al.]. Time- and Dose-Dependent Effects of 17 Beta-Estradiol on Short-Term, Real-Time Proliferation and Gene Expression in Porcine Granulosa Cells. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1139712
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1139712