Determination of phylogenetic typing of E.coli isolated from UTIs in many Hospitals of Baghdad city

Abstract EN

-Background: Escherichia coli is the major of causative agent in most of Urinary tract infections (UTIs), Phylogenetic analyses of E.coli isolated from urinary tract infections fall into four phylogenetic groups (A,B1, B2 and D), All Strains of these four groups differ in the characteristics of phenotype as antibiotic resistance , growth rate-temperature relationships and ability to use certain sugars.

Ami of study: To investigate the phylogenetic typing E.coli isolated from 660 urine samples from patients with UTIs in many hospitals of Baghdad city,as well as study the relationship between phylogenetic of E.coli with many important factors as gender; age and hospital wards.

Materials and Methods: From a total of six hundred - sixty urine samples were collected from UTIs patients in many hospitals (Baghdad/Iraq), through the period from March 2015 to September 2015 and inoculated on (MaCconckey and blood) agar, then incubated at 37 °C for 24 h.

,all isolates were identified by VITEK 2-GN system and Antimicrobial susceptibility pattern was determined by diffusion method (Kirby-Bauer disk) as well as the Phylogenetic grouping of the E.

coli isolates was determined by a multiplex polymerase chain reaction (PCR)technique.

Results: In this study, E.coli was isolated from 660 urine sample from UTIs patients; (400) isolated of E.coli strains , high percentage of E.

coli (41%) was isolated from AL-Yermouk hospital and (18.25%) form AL-Iskan hospital, whilst low percentage as (11.25%) , (13.75%), (14.75%) respectively from (Ghazi AL-Hariri , Iben Beledi and AL-Nooman) hospitals respectively.

Most of strains were isolated from female patients of AL-Yermouk hospital (31%) followed by (15% ) from AL-Iskan hospital , as well as AL-Yermouk hospital have high percentage in males (11% ) but close ratios in both hospitals (AL-Iskan and Ghazi AL-Hariri) hospitals respectively as (3.25 and 3)% ,whilst in ( Iben Beledi hospital and AL-Nooman ) hospitals were low ratio as (1 % ) and (0.5), so greater percentage (43%) of isolates in this study belonged to phylogroup B2 and (28.25, 24) % for both phylogroup D and A respectively, whilst low percentage (3.5%) for B1, so in this study show 1.25% was untypable as well as the isolation rate of E.coli were higher among age (11-20 ) years (31.75 %), but low percentage (19.25 &19 ) % in both age groups ( ≤10 and 21-32 ) years, so high Prevalence of most phylogenic type ( A, B2 and D ) as (7.25, 12.5 and 10.75) % respectively in age group (21-30) years, whilst (6%) of phylogenic B1in age group (41-50)years .