Comparison of Fecal Calprotectin Methods for Predicting Relapse of Pediatric Inflammatory Bowel Disease
Joint Authors
Kittanakom, Saranya
Shajib, Md. Sharif
Garvie, Kristine
Turner, Joceline
Brooks, Dan
Odeh, Sufian
Issenman, Robert
Chetty, V. Tony
Macri, Joseph
Khan, Waliul I.
Source
Canadian Journal of Gastroenterology and Hepatology
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-04-16
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background.
Pediatric inflammatory bowel disease (IBD) is on the rise worldwide.
Endoscopies are necessary for IBD assessment but are invasive, expensive, and inconvenient.
Recently, fecal calprotectin (FCal) was proposed as a noninvasive and specific marker of gut inflammation.
We evaluated the analytical performance of three FCal assays and their clinical performance in predicting relapse in pediatric IBD.
Methods.
This study used 40 pediatric IBD and 40 random non-IBD patients’ fecal samples.
Two automated ELISAs (Bühlmann and PhiCal® Calprotectin-EIA) and an EliA (Phadia 250 EliA-Calprotectin) were used to evaluate the analytical performance.
The clinical performance was assessed by PhiCal Calprotectin-EIA, EliA-Calprotectin, and Bühlmann immunochromatographic point-of-care test (POCT).
Results.
All assays displayed acceptable analytical performance below and above the medical decision cut-off [imprecision (CV < 10% intra-assay; <15% interassay); linearity (overall mean % deviation < 16.5%)].
The agreement with PhiCal Calprotectin-EIA was 100% and 78.6% for Bühlmann (95% CI, 87.5–100; Kappa: 1) and EliA-Calprotectin (95% CI, 60.5–89.8; Kappa: 0.32), respectively, and 63.6% between Bühlmann and EliA-Calprotectin (95% CI, 46.6–77.8; Kappa: 0.16).
All assays evaluated had similar clinical performance [AUC: 0.84 (EliA-Calprotectin); 0.83 (POCT and PhiCal Calprotectin-EIA)].
Conclusion.
FCal levels determined using the same method and assay together with clinical history would be a noninvasive and useful tool in monitoring pediatric IBD.
American Psychological Association (APA)
Kittanakom, Saranya& Shajib, Md. Sharif& Garvie, Kristine& Turner, Joceline& Brooks, Dan& Odeh, Sufian…[et al.]. 2017. Comparison of Fecal Calprotectin Methods for Predicting Relapse of Pediatric Inflammatory Bowel Disease. Canadian Journal of Gastroenterology and Hepatology،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1141345
Modern Language Association (MLA)
Kittanakom, Saranya…[et al.]. Comparison of Fecal Calprotectin Methods for Predicting Relapse of Pediatric Inflammatory Bowel Disease. Canadian Journal of Gastroenterology and Hepatology No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1141345
American Medical Association (AMA)
Kittanakom, Saranya& Shajib, Md. Sharif& Garvie, Kristine& Turner, Joceline& Brooks, Dan& Odeh, Sufian…[et al.]. Comparison of Fecal Calprotectin Methods for Predicting Relapse of Pediatric Inflammatory Bowel Disease. Canadian Journal of Gastroenterology and Hepatology. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1141345
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1141345