EGCG Maintains Th1Th2 Balance and Mitigates Ulcerative Colitis Induced by Dextran Sulfate Sodium through TLR4MyD88NF-κB Signaling Pathway in Rats
Joint Authors
Chen, Keyan
Bing, Xue
Linlang, Liang
Xuelei, Liu
Wanwei, Dong
Source
Canadian Journal of Gastroenterology and Hepatology
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-12-18
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Objective.
To observe the protective effect of epigallocatechin gallate (EGCG) on dextran sulfate sodium- (DSS-) induced ulcerative colitis in rats and to explore the roles of TLR4/MyD88/NF-κB signaling pathway.
Methods.
Rat models of ulcerative colitis were established by giving DSS.
EGCG (50 mg/kg/d) was given to assess disease activity index.
HE staining was applied to observe histological changes.
ELISA and qPCR detected the expression of inflammatory factors.
Flow cytometry was used to measure the percentage of CD4+IFN-γ+ and CD4+IL-4+ in the spleen and colon.
TLR4 antagonist E5564 was given in each group.
Flow cytometry was utilized to detect CD4+IFN-γ+ and CD4+IL-4+ cells.
Immunohistochemistry, qPCR, and western blot assay were applied to measure the expression of TLR4, MyD88, and NF-κB.
Results.
EGCG improved the intestinal mucosal injury in rats, inhibited production of inflammatory factors, maintained the balance of Th1/Th2, and reduced the expression of TLR4, MyD88, and NF-κB.
After TLR4 antagonism, the protective effect of EGCG on intestinal mucosal injury was weakened in rats with ulcerative colitis, and the expressions of inflammatory factors were upregulated.
Conclusion.
EGCG can inhibit the intestinal inflammatory response by reducing the severity of ulcerative colitis and maintaining the Th1/Th2 balance through the TLR4/MyD88/NF-κB signaling pathway.
American Psychological Association (APA)
Bing, Xue& Xuelei, Liu& Wanwei, Dong& Linlang, Liang& Chen, Keyan. 2017. EGCG Maintains Th1Th2 Balance and Mitigates Ulcerative Colitis Induced by Dextran Sulfate Sodium through TLR4MyD88NF-κB Signaling Pathway in Rats. Canadian Journal of Gastroenterology and Hepatology،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1141380
Modern Language Association (MLA)
Bing, Xue…[et al.]. EGCG Maintains Th1Th2 Balance and Mitigates Ulcerative Colitis Induced by Dextran Sulfate Sodium through TLR4MyD88NF-κB Signaling Pathway in Rats. Canadian Journal of Gastroenterology and Hepatology No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1141380
American Medical Association (AMA)
Bing, Xue& Xuelei, Liu& Wanwei, Dong& Linlang, Liang& Chen, Keyan. EGCG Maintains Th1Th2 Balance and Mitigates Ulcerative Colitis Induced by Dextran Sulfate Sodium through TLR4MyD88NF-κB Signaling Pathway in Rats. Canadian Journal of Gastroenterology and Hepatology. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1141380
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1141380