Detection of Methicillin Resistant Staphylococcus aureus and Determination of Minimum Inhibitory Concentration of Vancomycin for Staphylococcus aureus Isolated from PusWound Swab Samples of the Patients Attending a Tertiary Care Hospital in Kathmandu, Nepal
Joint Authors
Pant, Narayan Dutt
Adhikari, Raghabendra
Neupane, Sanjeev
Neupane, Mukesh
Bhattarai, Roshan
Bhatta, Sabita
Chaudhary, Raina
Lekhak, Binod
Source
Canadian Journal of Infectious Diseases and Medical Microbiology
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-05
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
The present study was conducted to evaluate the performance of cefoxitin disc diffusion method and oxacillin broth microdilution method for detection of methicillin resistant S.
aureus (MRSA), taking presence of mecA gene as reference.
In addition, inducible clindamycin resistance and beta-lactamase production were studied and minimum inhibitory concentration (MIC) of vancomycin for S.
aureus isolates was determined.
A total of 711 nonrepeated pus/wound swab samples from different anatomic locations were included in the study.
The Staphylococcus aureus was identified on the basis of colony morphology, Gram’s stain, and biochemical tests.
A total of 110 (15.47%) S.
aureus isolates were recovered, of which 39 (35.50%) isolates were identified as MRSA by cefoxitin disc diffusion method.
By oxacillin broth microdilution method, 31.82% of the Staphylococcus aureus isolates were found to be MRSA.
However, mecA gene was present in only 29.1% of the isolates.
Further, beta-lactamase production was observed in 71.82% of the isolates, while inducible clindamycin resistance was found in 10% of S.
aureus isolates.
The MIC value of vancomycin for S.
aureus ranged from 0.016 μg/mL to 1 μg/mL.
On the basis of the absolute sensitivity (100%), both phenotypic methods could be employed for routine diagnosis of MRSA in clinical microbiology laboratory; however cefoxitin disc diffusion could be preferred over MIC method considering time and labour factor.
American Psychological Association (APA)
Adhikari, Raghabendra& Pant, Narayan Dutt& Neupane, Sanjeev& Neupane, Mukesh& Bhattarai, Roshan& Bhatta, Sabita…[et al.]. 2017. Detection of Methicillin Resistant Staphylococcus aureus and Determination of Minimum Inhibitory Concentration of Vancomycin for Staphylococcus aureus Isolated from PusWound Swab Samples of the Patients Attending a Tertiary Care Hospital in Kathmandu, Nepal. Canadian Journal of Infectious Diseases and Medical Microbiology،Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1141540
Modern Language Association (MLA)
Adhikari, Raghabendra…[et al.]. Detection of Methicillin Resistant Staphylococcus aureus and Determination of Minimum Inhibitory Concentration of Vancomycin for Staphylococcus aureus Isolated from PusWound Swab Samples of the Patients Attending a Tertiary Care Hospital in Kathmandu, Nepal. Canadian Journal of Infectious Diseases and Medical Microbiology No. 2017 (2017), pp.1-6.
https://search.emarefa.net/detail/BIM-1141540
American Medical Association (AMA)
Adhikari, Raghabendra& Pant, Narayan Dutt& Neupane, Sanjeev& Neupane, Mukesh& Bhattarai, Roshan& Bhatta, Sabita…[et al.]. Detection of Methicillin Resistant Staphylococcus aureus and Determination of Minimum Inhibitory Concentration of Vancomycin for Staphylococcus aureus Isolated from PusWound Swab Samples of the Patients Attending a Tertiary Care Hospital in Kathmandu, Nepal. Canadian Journal of Infectious Diseases and Medical Microbiology. 2017. Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1141540
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1141540