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Laboratory Diagnosis of Malaria: Comparison of Manual and Automated Diagnostic Tests
Joint Authors
Shamsi, Tahir
Mukry, Samina Naz
Saud, Madiha
Sufaida, Gul
Shaikh, Kashif
Naz, Arshi
Source
Canadian Journal of Infectious Diseases and Medical Microbiology
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-04-05
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Malaria is the second most prevalent disease in Pakistan resulting in ~30,000 annual deaths.
In endemic countries like Pakistan precise and timely diagnosis of malaria is imperative to overcome the associated risks of fatal outcomes.
Malarial parasite was screened in 128 malaria suspected patients and 150 healthy controls, by species-specific PCR, microscopy of blood smears, hemoanalyzer Sysmex XE-2100, and rapid test devices (First Response Malaria® and ICT Malaria Combo®).
The microscopy detected MP in 126 samples (parasite load/µl 386–53712/µl); 71.094% were infected with Plasmodium vivax and 14.844% with P.
falciparum while 14.062% had mixed P.
vivax and P.
falciparum infection.
The mean parasite load for P.
vivax and P.
falciparum was 14496/µl and 24410/µl, respectively.
The abnormal scattergrams of DIFF, WBC/ Baso, IMI channel, and RET-EXT on Sysmex XE-2100 supported 99.2% parasite detection, whereas only 93% of confirmed malaria cases were detected by both rapid tests.
About 127 samples were positive by PCR.
Since Sysmex XE-2100 automatically detected the presence of malarial parasite with high sensitivity, it can be a good option for presumptive diagnosis in endemic areas.
Microscopy remains the gold standard to confirm MP in suspected patients.
Rapid diagnostic tests have acceptable sensitivity and specificity.
American Psychological Association (APA)
Mukry, Samina Naz& Saud, Madiha& Sufaida, Gul& Shaikh, Kashif& Naz, Arshi& Shamsi, Tahir. 2017. Laboratory Diagnosis of Malaria: Comparison of Manual and Automated Diagnostic Tests. Canadian Journal of Infectious Diseases and Medical Microbiology،Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1141628
Modern Language Association (MLA)
Mukry, Samina Naz…[et al.]. Laboratory Diagnosis of Malaria: Comparison of Manual and Automated Diagnostic Tests. Canadian Journal of Infectious Diseases and Medical Microbiology No. 2017 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-1141628
American Medical Association (AMA)
Mukry, Samina Naz& Saud, Madiha& Sufaida, Gul& Shaikh, Kashif& Naz, Arshi& Shamsi, Tahir. Laboratory Diagnosis of Malaria: Comparison of Manual and Automated Diagnostic Tests. Canadian Journal of Infectious Diseases and Medical Microbiology. 2017. Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1141628
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1141628