PET Imaging of FSHR Expression in Tumors with 68Ga-Labeled FSH1 Peptide
Joint Authors
Liu, Guifeng
Yang, Min
Xu, Yuping
Pan, Donghui
Wang, Yanting
Yue, Yuanyuan
Wang, Lizhen
Yan, Junjie
Wang, Xinyu
Yang, Runlin
Source
Contrast Media & Molecular Imaging
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-08-23
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
FSHR is an appealing target for cancer theranostics.
Radiolabeled FSH1 and its derivatives have shown potential to in vivo detect FSHR expression.
However, moderate labeling yields (~50% nondecay-corrected) may partially limit their wide use.
68Ga is an excellent PET nuclide due to availability, nearly quantitative reaction, and short physical half-life.
In this study, 68Ga labeled FSH1 peptide was developed for imaging of FSHR in cancers.
In vitro studies and MicroPET imaging were performed in PC-3 prostate tumor model.
[68Ga] Ga-NOTA-MAL-FSH1 can be produced within 20 min with 93.2±2.1% yield and the radiochemical purity was greater than 95%.
It showed that [68Ga] Ga-NOTA-MAL-FSH1 possessed FSHR binding affinities.
The tracer was stable in PBS and human serum for at least 2 hours.
MicroPET imaging revealed that the PC-3 xenografts were clearly visualized and the tumor uptakes were 1.87±0.10, 1.26±0.06, and 0.71±0.10% ID/g at 0.5, 1 h, and 2 h postinjection.
The corresponding tumor to blood and tumor to muscle ratios were 1.77±0.70, 7.94±1.35, and 10.37±1.16 and 7.42±0.46, 26.13±2.99, and 36.40±2.54, respectively.
FSHR binding specificity was also demonstrated by reduced tumor uptake of [68Ga] Ga-NOTA-MAL-FSH1 after coinjecting excess unlabeled FSH1 peptide.
The favorable characters of [68Ga] Ga-NOTA-MAL-FSH1 such as convenient synthesis and specific tumor uptake warrant its further investigation for FSHR expression imaging.
American Psychological Association (APA)
Pan, Donghui& Liu, Guifeng& Xu, Yuping& Wang, Yanting& Yue, Yuanyuan& Wang, Lizhen…[et al.]. 2017. PET Imaging of FSHR Expression in Tumors with 68Ga-Labeled FSH1 Peptide. Contrast Media & Molecular Imaging،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1141697
Modern Language Association (MLA)
Pan, Donghui…[et al.]. PET Imaging of FSHR Expression in Tumors with 68Ga-Labeled FSH1 Peptide. Contrast Media & Molecular Imaging No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1141697
American Medical Association (AMA)
Pan, Donghui& Liu, Guifeng& Xu, Yuping& Wang, Yanting& Yue, Yuanyuan& Wang, Lizhen…[et al.]. PET Imaging of FSHR Expression in Tumors with 68Ga-Labeled FSH1 Peptide. Contrast Media & Molecular Imaging. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1141697
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1141697