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Fibered Confocal Fluorescence Microscopy for the Noninvasive Imaging of Langerhans Cells in Macaques
Joint Authors
Todorova, Biliana
Salabert, Nina
Tricot, Sabine
Boisgard, Raphaël
Rathaux, Mélanie
Le Grand, Roger
Chapon, Catherine
Source
Contrast Media & Molecular Imaging
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-06-07
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Purpose.
We developed a new approach to visualize skin Langerhans cells by in vivo fluorescence imaging in nonhuman primates.
Procedures.
Macaques were intradermally injected with a monoclonal, fluorescently labeled antibody against HLA-DR molecule and were imaged for up to 5 days by fibered confocal microscopy (FCFM).
Results.
The network of skin Langerhans cells was visualized by in vivo fibered confocal fluorescence microscopy.
Quantification of Langerhans cells revealed no changes to cell density with time.
Ex vivo experiments confirmed that injected fluorescent HLA-DR antibody specifically targeted Langerhans cells in the epidermis.
Conclusions.
This study demonstrates the feasibility of single-cell, in vivo imaging as a noninvasive technique to track Langerhans cells in nontransgenic animals.
American Psychological Association (APA)
Todorova, Biliana& Salabert, Nina& Tricot, Sabine& Boisgard, Raphaël& Rathaux, Mélanie& Le Grand, Roger…[et al.]. 2017. Fibered Confocal Fluorescence Microscopy for the Noninvasive Imaging of Langerhans Cells in Macaques. Contrast Media & Molecular Imaging،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1141715
Modern Language Association (MLA)
Todorova, Biliana…[et al.]. Fibered Confocal Fluorescence Microscopy for the Noninvasive Imaging of Langerhans Cells in Macaques. Contrast Media & Molecular Imaging No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1141715
American Medical Association (AMA)
Todorova, Biliana& Salabert, Nina& Tricot, Sabine& Boisgard, Raphaël& Rathaux, Mélanie& Le Grand, Roger…[et al.]. Fibered Confocal Fluorescence Microscopy for the Noninvasive Imaging of Langerhans Cells in Macaques. Contrast Media & Molecular Imaging. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1141715
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1141715