Fibered Confocal Fluorescence Microscopy for the Noninvasive Imaging of Langerhans Cells in Macaques

Abstract EN

Purpose.

We developed a new approach to visualize skin Langerhans cells by in vivo fluorescence imaging in nonhuman primates.

Procedures.

Macaques were intradermally injected with a monoclonal, fluorescently labeled antibody against HLA-DR molecule and were imaged for up to 5 days by fibered confocal microscopy (FCFM).

Results.

The network of skin Langerhans cells was visualized by in vivo fibered confocal fluorescence microscopy.

Quantification of Langerhans cells revealed no changes to cell density with time.

Ex vivo experiments confirmed that injected fluorescent HLA-DR antibody specifically targeted Langerhans cells in the epidermis.

Conclusions.

This study demonstrates the feasibility of single-cell, in vivo imaging as a noninvasive technique to track Langerhans cells in nontransgenic animals.