One-Pot Aqueous Synthesis of Fluorescent Ag-In-Zn-S Quantum DotPolymer Bioconjugates for Multiplex Optical Bioimaging of Glioblastoma Cells
Joint Authors
Mansur, H. S.
Mansur, Alexandra A. P.
Carvalho, Sandhra M.
Caires, Anderson J.
Source
Contrast Media & Molecular Imaging
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-11-13
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Cancer research has experienced astonishing advances recently, but cancer remains a major threat because it is one of the leading causes of death worldwide.
Glioblastoma (GBM) is the most malignant brain tumor, where the early diagnosis is vital for longer survival.
Thus, this study reports the synthesis of novel water-dispersible ternary AgInS2 (AIS) and quaternary AgInS2-ZnS (ZAIS) fluorescent quantum dots using carboxymethylcellulose (CMC) as ligand for multiplexed bioimaging of malignant glioma cells (U-87 MG).
Firstly, AgInS2 core was prepared using a one-pot aqueous synthesis stabilized by CMC at room temperature and physiological pH.
Then, an outer layer of ZnS was grown and thermally annealed to improve their optical properties and split the emission range, leading to core-shell alloyed nanostructures.
Their physicochemical and optical properties were characterized, demonstrating that luminescent monodispersed AIS and ZAIS QDs were produced with average sizes of 2.2 nm and 4.3 nm, respectively.
Moreover, the results evidenced that they were cytocompatible using in vitro cell viability assays towards human embryonic kidney cell line (HEK 293T) and U-87 MG cells.
These AIS and ZAIS successfully behaved as fluorescent nanoprobes (red and green, resp.) allowing multiplexed bioimaging and biolabeling of costained glioma cells using confocal microscopy.
American Psychological Association (APA)
Mansur, Alexandra A. P.& Mansur, H. S.& Carvalho, Sandhra M.& Caires, Anderson J.. 2017. One-Pot Aqueous Synthesis of Fluorescent Ag-In-Zn-S Quantum DotPolymer Bioconjugates for Multiplex Optical Bioimaging of Glioblastoma Cells. Contrast Media & Molecular Imaging،Vol. 2017, no. 2017, pp.1-15.
https://search.emarefa.net/detail/BIM-1141728
Modern Language Association (MLA)
Mansur, Alexandra A. P.…[et al.]. One-Pot Aqueous Synthesis of Fluorescent Ag-In-Zn-S Quantum DotPolymer Bioconjugates for Multiplex Optical Bioimaging of Glioblastoma Cells. Contrast Media & Molecular Imaging No. 2017 (2017), pp.1-15.
https://search.emarefa.net/detail/BIM-1141728
American Medical Association (AMA)
Mansur, Alexandra A. P.& Mansur, H. S.& Carvalho, Sandhra M.& Caires, Anderson J.. One-Pot Aqueous Synthesis of Fluorescent Ag-In-Zn-S Quantum DotPolymer Bioconjugates for Multiplex Optical Bioimaging of Glioblastoma Cells. Contrast Media & Molecular Imaging. 2017. Vol. 2017, no. 2017, pp.1-15.
https://search.emarefa.net/detail/BIM-1141728
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1141728