Comparative Evaluation of Anti-HER2 Affibody Molecules Labeled with 64Cu Using NOTA and NODAGA
Joint Authors
Orlova, Anna
Tolmachev, Vladimir
Yim, Cheng-Bin
Rajander, Johan
Perols, Anna
Karlström, Amelie Eriksson
Haaparanta-Solin, Merja
Grönroos, Tove J.
Solin, Olof
Source
Contrast Media & Molecular Imaging
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-02-28
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Imaging using affibody molecules enables discrimination between breast cancer metastases with high and low expression of HER2, making appropriate therapy selection possible.
This study aimed to evaluate if the longer half-life of 64Cu (T1/2 = 12.7 h) would make 64Cu a superior nuclide compared to 68Ga for PET imaging of HER2 expression using affibody molecules.
The synthetic ZHER2:S1 affibody molecule was conjugated with the chelators NOTA or NODAGA and labeled with 64Cu.
The tumor-targeting properties of 64Cu-NOTA-ZHER2:S1 and 64Cu-NODAGA-ZHER2:S1 were evaluated and compared with the targeting properties of 68Ga-NODAGA-ZHER2:S1 in mice.
Both 64Cu-NOTA-ZHER2:S1 and 64Cu-NODAGA-ZHER2:S1 demonstrated specific targeting of HER2-expressing xenografts.
At 2 h after injection of 64Cu-NOTA-ZHER2:S1, 64Cu-NODAGA-ZHER2:S1, and 68Ga-NODAGA-ZHER2:S1, tumor uptakes did not differ significantly.
Renal uptake of 64Cu-labeled conjugates was dramatically reduced at 6 and 24 h after injection.
Notably, radioactivity uptake concomitantly increased in blood, lung, liver, spleen, and intestines, which resulted in decreased tumor-to-organ ratios compared to 2 h postinjection.
Organ uptake was lower for 64Cu-NODAGA-ZHER2:S1.
The most probable explanation for this biodistribution pattern was the release and redistribution of renal radiometabolites.
In conclusion, monoamide derivatives of NOTA and NODAGA may be suboptimal chelators for radiocopper labeling of anti-HER2 affibody molecules and, possibly, other scaffold proteins with high renal uptake.
American Psychological Association (APA)
Tolmachev, Vladimir& Yim, Cheng-Bin& Rajander, Johan& Perols, Anna& Karlström, Amelie Eriksson& Haaparanta-Solin, Merja…[et al.]. 2017. Comparative Evaluation of Anti-HER2 Affibody Molecules Labeled with 64Cu Using NOTA and NODAGA. Contrast Media & Molecular Imaging،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1141858
Modern Language Association (MLA)
Tolmachev, Vladimir…[et al.]. Comparative Evaluation of Anti-HER2 Affibody Molecules Labeled with 64Cu Using NOTA and NODAGA. Contrast Media & Molecular Imaging No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1141858
American Medical Association (AMA)
Tolmachev, Vladimir& Yim, Cheng-Bin& Rajander, Johan& Perols, Anna& Karlström, Amelie Eriksson& Haaparanta-Solin, Merja…[et al.]. Comparative Evaluation of Anti-HER2 Affibody Molecules Labeled with 64Cu Using NOTA and NODAGA. Contrast Media & Molecular Imaging. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1141858
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1141858