Use of Cyclic Backbone NGR-Based SPECT to Increase Efficacy of Postmyocardial Infarction Angiogenesis Imaging
Joint Authors
Post, Mark J.
Bauwens, Matthias
Hendrikx, Geert
Hackeng, Tilman M.
van Gorp, Rick
Schurgers, Leon J.
Mottaghy, Felix M.
Dijkgraaf, Ingrid
Source
Contrast Media & Molecular Imaging
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-10-24
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
As CD13 is selectively expressed in angiogenesis, it can serve as a target for molecular imaging tracers to noninvasively visualize angiogenic processes in vivo.
The CD13-targeting moiety NGR was synthesized and cyclized by native chemical ligation (NCL) instead of disulfide bridging, leading to a cyclic peptide backbone: cyclo(Cys-Asn-Gly-Arg-Gly) (coNGR).
Beside this new monomeric coNGR, a tetrameric NGR peptide co(NGR)4 was designed and synthesized.
After radiolabeling, their in vitro and in vivo characteristics were determined.
Both coNGR-based imaging agents displayed considerably higher standardized uptake values (SUVs) at infarcted areas compared to the previously reported disulfide-cyclized cNGR imaging agent.
Uptake patterns of 111In-coNGR and 111In-co(NGR)4 coincided with CD13 immunohistochemistry on excised hearts.
Blood stability tests indicated better stability for both novel imaging agents after 50 min blood incubation compared to the disulfide-cyclized cNGR imaging agent.
In mice, both coNGR peptides cleared rapidly from the blood mainly via the kidneys.
In addition, co(NGR)4 showed a significantly higher specific uptake in infarcted myocardium compared to coNGR and thus is a promising sensitive imaging agent for detection of angiogenesis in infarcted myocardium.
American Psychological Association (APA)
Hendrikx, Geert& Hackeng, Tilman M.& van Gorp, Rick& Bauwens, Matthias& Schurgers, Leon J.& Mottaghy, Felix M.…[et al.]. 2017. Use of Cyclic Backbone NGR-Based SPECT to Increase Efficacy of Postmyocardial Infarction Angiogenesis Imaging. Contrast Media & Molecular Imaging،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1141865
Modern Language Association (MLA)
Hendrikx, Geert…[et al.]. Use of Cyclic Backbone NGR-Based SPECT to Increase Efficacy of Postmyocardial Infarction Angiogenesis Imaging. Contrast Media & Molecular Imaging No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1141865
American Medical Association (AMA)
Hendrikx, Geert& Hackeng, Tilman M.& van Gorp, Rick& Bauwens, Matthias& Schurgers, Leon J.& Mottaghy, Felix M.…[et al.]. Use of Cyclic Backbone NGR-Based SPECT to Increase Efficacy of Postmyocardial Infarction Angiogenesis Imaging. Contrast Media & Molecular Imaging. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1141865
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1141865