P27 Promotes TGF-β-Mediated Pulmonary Fibrosis via Interacting with MTORC2
Joint Authors
Xu, Zhihao
Dai, Yu-heng
Li, Xiao-qing
Dong, Da-peng
Gu, Hai-bo
Kong, Cheng-ying
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-09-19
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Pulmonary fibrosis (PF), a progressive and life-threatening pulmonary disease, is the main pathological basis of interstitial lung disease (ILD) which includes the idiopathic pulmonary fibrosis (IPF).
No effective therapeutic strategy for pulmonary fibrosis has been established.
TGF-β signaling has emerged as the vital regulator of PF; however, the detailed molecular mechanisms of TGF-β in PF were uncertain.
In the present study, we proved that inhibition of MTORC2 suppresses the expression of P27 in MRC5 and HLF cells.
And in bleomycin-induced PF model, the expression of α-SMA and P27 was upregulated.
Moreover, TGF-β application increased the level of α-SMA, vimentin, and P27 in MRC5 and HLF cells.
Furthermore, P27 overexpression advanced the cell cycle process and promoted the proliferation of MRC5 and HLF cells.
Finally, the rescue experiment showed that MTORC2 knockdown reversed P27 overexpression-induced cell cycle acceleration and proliferation.
Thus, our results suggest that P27 is involved in TGF-β-mediated PF, which was regulated by MTORC2, providing a novel insight into the development of PF.
American Psychological Association (APA)
Dai, Yu-heng& Li, Xiao-qing& Dong, Da-peng& Gu, Hai-bo& Kong, Cheng-ying& Xu, Zhihao. 2019. P27 Promotes TGF-β-Mediated Pulmonary Fibrosis via Interacting with MTORC2. Canadian Respiratory Journal،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1145780
Modern Language Association (MLA)
Dai, Yu-heng…[et al.]. P27 Promotes TGF-β-Mediated Pulmonary Fibrosis via Interacting with MTORC2. Canadian Respiratory Journal No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1145780
American Medical Association (AMA)
Dai, Yu-heng& Li, Xiao-qing& Dong, Da-peng& Gu, Hai-bo& Kong, Cheng-ying& Xu, Zhihao. P27 Promotes TGF-β-Mediated Pulmonary Fibrosis via Interacting with MTORC2. Canadian Respiratory Journal. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1145780
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1145780