Circ_MDM2_000139, Circ_ATF2_001418, Circ_CDC25C_002079, and Circ_BIRC6_001271 Are Involved in the Functions of XAV939 in Non-Small Cell Lung Cancer
Joint Authors
Yu, Haixiang
Xu, Lei
Liu, Zhengjia
Guo, Bo
Han, Zhifeng
Xin, Hua
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-11-27
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Background.
The small molecule inhibitor XAV939 could inhibit the proliferation and promote the apoptosis of non-small cell lung cancer (NSCLC) cells.
This study was conducted to identify the key circular RNAs (circRNAs) and microRNAs (miRNAs) in XAV939-treated NSCLC cells.
Methods.
After grouping, the NCL-H1299 cells in the treatment group were treated by 10 μM XAV939 for 12 h.
RNA-sequencing was performed, and then the differentially expressed circRNAs (DE-circRNAs) were analyzed by the edgeR package.
Using the clusterprofiler package, enrichment analysis for the hosting genes of the DE-circRNAs was performed.
Using Cytoscape software, the miRNA-circRNA regulatory network was built for the disease-associated miRNAs and the DE-circRNAs.
The DE-circRNAs that could translate into proteins were predicted using circBank database and IRESfinder tool.
Finally, the transcription factor (TF)-circRNA regulatory network was built by Cytoscape software.
In addition, A549 and HCC-827 cell treatment with XAV939 were used to verify the relative expression levels of key DE-circRNAs.
Results.
There were 106 DE-circRNAs (including 61 upregulated circRNAs and 45 downregulated circRNAs) between treatment and control groups.
Enrichment analysis for the hosting genes of the DE-circRNAs showed that ATF2 was enriched in the TNF signaling pathway.
Disease association analysis indicated that 8 circRNAs (including circ_MDM2_000139, circ_ATF2_001418, circ_CDC25C_002079, and circ_BIRC6_001271) were correlated with NSCLC.
In the miRNA-circRNA regulatory network, let-7 family members⟶circ_MDM2_000139, miR-16-5p/miR-134-5p⟶circ_ATF2_001418, miR-133b⟶circ_BIRC6_001271, and miR-221-3p/miR-222-3p⟶circ_CDC25C_002079 regulatory pairs were involved.
A total of 47 DE-circRNAs could translate into proteins.
Additionally, circ_MDM2_000139 was targeted by the TF POLR2A.
The verification test showed that the relative expression levels of circ_MDM2_000139, circ_CDC25C_002079, circ_ATF2_001418, and circ_DICER1_000834 in A549 and HCC-827 cell treatment with XAV939 were downregulated comparing with the control.
Conclusions.
Let-7 family members and POLR2A targeting circ_MDM2_000139, miR-16-5p/miR-134-5p targeting circ_ATF2_001418, miR-133b targeting circ_BIRC6_001271, and miR-221-3p/miR-222-3p targeting circ_CDC25C_002079 might be related to the mechanism in the treatment of NSCLC by XAV939.
American Psychological Association (APA)
Yu, Haixiang& Xu, Lei& Liu, Zhengjia& Guo, Bo& Han, Zhifeng& Xin, Hua. 2019. Circ_MDM2_000139, Circ_ATF2_001418, Circ_CDC25C_002079, and Circ_BIRC6_001271 Are Involved in the Functions of XAV939 in Non-Small Cell Lung Cancer. Canadian Respiratory Journal،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1145814
Modern Language Association (MLA)
Yu, Haixiang…[et al.]. Circ_MDM2_000139, Circ_ATF2_001418, Circ_CDC25C_002079, and Circ_BIRC6_001271 Are Involved in the Functions of XAV939 in Non-Small Cell Lung Cancer. Canadian Respiratory Journal No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1145814
American Medical Association (AMA)
Yu, Haixiang& Xu, Lei& Liu, Zhengjia& Guo, Bo& Han, Zhifeng& Xin, Hua. Circ_MDM2_000139, Circ_ATF2_001418, Circ_CDC25C_002079, and Circ_BIRC6_001271 Are Involved in the Functions of XAV939 in Non-Small Cell Lung Cancer. Canadian Respiratory Journal. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1145814
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1145814